Triple-negative　breast　cancer　is　particularly　difficultto　treatbecause　of　its　high　degree　of　malignancy　and　poor　prognosis.　A　fluorescenceresonance　energy　transfer　(FRET)　nanoplatform　plays　a　very　importantrole　in　disease　diagnosis　and　treatment　due　to　its　unique　detectionperformance.　Combining　the　properties　of　agglomeration-induced　emissionfluorophore　and　FRET　pair,　a　FRET　nanoprobe　(HMSN/DOX/RVRR/PAMAM/TPE)induced　by　specific　cleavage　was　designed.　First,　hollow　mesoporoussilica　nanoparticles　(HMSNs)　were　used　as　drug　carriers　to　load　doxorubicin(DOX).　HMSN　nanopores　were　coated　with　the　RVRR　peptide.　Then,　polyamylamine/phenylethane(PAMAM/TPE)　was　combined　in　the　outermost　layer.　When　Furin　cut　offthe　RVRR　peptide,　DOX　was　released　and　adhered　to　PAMAM/TPE.　Finally,the　TPE/DOX　FRET　pair　was　constituted.　The　overexpression　of　Furinin　the　triple-negative　breast　cancer　cell　line　(MDA-MB-468　cell)　canbe　quantitatively　detected　by　FRET　signal　generation,　so　as　to　monitorcell　physiology.　In　conclusion,　the　HMSN/DOX/RVRR/PAMAM/TPE　nanoprobeswere　designed　to　provide　a　new　idea　for　the　quantitative　detectionof　Furin　and　drug　delivery,　which　is　conducive　to　the　early　diagnosisand　treatment　of　triple-negative　breast　cancer.