The　emission　of　persistent　luminescence　nanoparticles　(PLNPs)　is　in　the　second　near　infrared　region　(NIR-II,　1000-1700　nm),　considered　as　ideal　fluorescence　imaging　materials　because　of　their　special　characteristic　consist　of　no　autofluorescence,　weak　scattering　and　deep　tissue　penetration　depth　in　biological　tissues.　However,　it　is　still　a　great　challenge　to　enhance　their　persistent　luminescence　intensity.　Herein,　we　reported　a　new　MgGe0.8-Ga0.2O3:　Yb3+　(MGGO:　Yb3+)　PLNPs,　which　were　synthesized　through　solvothermal　liquid-solid-solution　and　salt　microemulsion　method.　We　found　that　partially　replacing　Ge4+　with　Ga3+　ions　in　MgGeO3　host　can　increase　the　density　of　traps,　thus　significantly　improving　the　NIR-II　persistent　luminescence　intensity　of　Yb3+　ions　(the　decay　time　is　more　than　33　min).　Moreover,　X-ray　could　be　used　to　repeatedly　stimulate　the　NIR-II　persistent　emission　of　MGGO:　Yb3+,　which　is　pivotal　for　deep　tissues　long　term　imaging.　The　optical　penetration　length　of　Yb3+　ions　persistent　luminescence　was　assessed　to　be　3.9　mm　via　capping　tissue.　The　above　results　reveal　that　MGGO:　Yb3+　PLNPs　are　promising　material　to　be　applied　to　deep-tissue　imaging　field.