To　improve　the　fabrication　efficiency　of　the　two-photon　polymerization　(TPP)　laser　direct　writing,　the　TPP　exposure　process　was　set　to　complete　by　a　single-line　scan,　which　was　named　2D　TPP.　The　voxel　of　the　2D　TPP　should　be　large　enough　to　cross　the　photoresist　and　the　underlayer.　To　explore　the　resolution　limit　of　the　2D　TPP　considering　the　thickness　of　the　photoresist,　a　new　method　named　the　45°　scanning　method　was　proposed.　Meanwhile,　a　two-photon　micro-nano　fabrication　platform　was　developed.　A　group　of　experiments　based　on　the　orthogonal　decomposition　method　was　carried　out　to　analyze　the　width　and　length　of　the　voxel　on　the　S1805　photoresist　under　different　laser　power　and　processing　speed.　To　confirm　whether　the　resolution　of　the　micro-nano　structures　fabricated　by　2D　TPP　is　consistent　with　the　width　of　the　voxel,　aluminum　wire　grids　were　fabricated　through　the　2D　TPP　and　the　metal　lift-off　process.　A　second-order　regression　equation　of　the　machining　resolution　and　input　parameters　of　the　2D　TPP　is　deduced.　The　correlation　coefficient　between　the　width　of　the　voxel　and　the　aluminum　wire　grids　is　0.961,　which　means　a　significant　positive　correlation　between　them.　Finally,　the　second-order　regression　model　derived　from　the　fabrication　resolution　of　the　2D　TPP　was　validated　by　experiments.　Full　2D　grids　were　fabricated　using　2D　TPP　and　mental　lift-off　process.　This　paper　provides　a　convenient,　low-cost,　and　high-efficiency　method　for　calibrating　the　fabrication　resolution　of　2D　TPP　on　various　photoresists.　©　2023　by　the　authors.