The　surface　modification　strategy　is　widely　used　to　solve　the　problems　of　low　stability,　agglomeration,　surface　oxidation　and　photoluminescence　quenching　of　quantum　dots　(QDs)　in　practical　applications.　However　this　method　can　easily　destroys　the　surface　ligands　of　QDs,　increases　defects　even　leads　to　a　huge　loss　of　fluorescence.　In　order　to　improve　the　stability　of　QDs,　a　new　synthesis　method　of　QD-silica　hybrid　nanospheres　was　proposed　in　this　study.　These　QD-silica　hybrid　nanospheres　are　characterized　by　using　mesoporous　silica　spheres　(MSSs)　as　template,　adsorbing　QDs　as　one　shell,　and　then　coating　a　silica　layer　as　another　shell　(named　SQS).　The　template　MSSs　were　functionalized　by　(3-mercaptopropyl)　trimethoxysilane　(MPTMS)　in　order　to　connect　MSSs　and　QDs.　After　that,　the　QD-adsorbed　silica　spheres　were　coated　with　silica　as　the　encapsulation　layer　by　Stober　method.　The　structure　and　morphology　of　SQS　were　analyzed　by　TEM.　The　effects　of　different　contents　of　MPTMS　and　tetraethoxysilane(TEOS)　were　experimentally　compared.　Finally,　it　was　found　that　the　optimal　contents　of　MPTMS　and　TEOS　was　250μL　and　1.5mL,　respectively.　The　luminescence　intensity　of　SQS　samples　could　reach　2　times　higher　than　that　of　pure　QD　solution.　Meanwhile,　SQS　hybrid　nanospheres　could　avoid　the　tiny　spots　inside　the　microstructure　caused　by　QD　aggregation　and　play　a　better　role　in　dispersion.　©　2022　SPIE.