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author:

Wang, Jingtong (Wang, Jingtong.) [1] | Cao, Yongqiang (Cao, Yongqiang.) [2] | Li, Zhao (Li, Zhao.) [3] | Dong, Meiling (Dong, Meiling.) [4] | Dou, Wei (Dou, Wei.) [5] | Xu, Xiaoping (Xu, Xiaoping.) [6] (Scholars:许小平) | He, Shengbin (He, Shengbin.) [7]

Indexed by:

EI SCIE

Abstract:

Rapid and sensitive quantification of pathogenic bacteria is highly desired for environmental health supervision and food safety control. Yet, the amplification and detection of bacteria with a concentration lower than 10(2) cfu mL(-1) remains a great challenge. Here, we combined an allosteric aptamer (AAP) with a gold nanoparticle (AuNP) for assembling a bridge-DNA synthesis system (named as AuNP-BDS) to amplify the bacterial signals. The AAP and its paired primer (PP) were covalently linked to two different AuNPs, respectively: one named as AAP-AuNP and the other PP-AuNP. Upon recognition of the antigen from the pathogenic bacteria, AAP alters its conformation to initiate DNA synthesis on the AuNP surface. The DNA products from AAP-AuNP and PP-AuNP form bridges to each other through base pairing, resulting in the aggregation and colorimetric response of the AuNPs. By using E. coli O157:H7 as an example, the AuNP-BDS could quantify pathogenic bacteria in water with a concentration as low as 10 cfu mL(-1) within 60 min and without any enrichment. The colorimetric response values of AuNP-BDS were found to be linearly related to the bacterial concentrations in the range of 10 to 10(3) cfu mL(-1). Good practicability of the AuNP-BDS in quantifying E. coli O157:H7 from tap water, juices, and milks was demonstrated. The AuNP-BDS could be exploited to facilitate the rapid and sensitive quantification of pathogenic bacteria for food safety control.

Keyword:

Community:

  • [ 1 ] [Wang, Jingtong]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China
  • [ 2 ] [Cao, Yongqiang]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China
  • [ 3 ] [Li, Zhao]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China
  • [ 4 ] [Dong, Meiling]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China
  • [ 5 ] [Dou, Wei]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China
  • [ 6 ] [He, Shengbin]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China
  • [ 7 ] [Xu, Xiaoping]Fuzhou Univ, Coll Chem, Fuzhou 350108, Fujian, Peoples R China

Reprint 's Address:

  • [He, Shengbin]Guangxi Med Univ, Sch Basic Med Sci, Guangxi Coll & Univ Key Lab Biol Mol Med Res, Key Lab Longev & Aging related Dis,Chinese Minist, Nanning 530021, Guangxi, Peoples R China;;

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Source :

ANALYTICAL METHODS

ISSN: 1759-9660

Year: 2022

Issue: 3

Volume: 15

Page: 275-283

3 . 1

JCR@2022

2 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:74

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count: 2

SCOPUS Cited Count: 4

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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