As　a　dimeric　protein,　thyroglobulin　(Tg)　is　an　important　biomarker　for　different　thyroid　cancer　(DTC),　so　designing　effective　method　to　detect　Tg　is　of　great　significance.　In　this　work,　by　preparing　beta-cyclodextrin　(CD)　functionalized　carbon　nanotubes　(CNTs)　nanohybrid　(CD-CNTs)　as　carrier　to　immobilize　primary　antibody　(Ab(1))　of　Tg,　assembling　sulfydryl　ferrocene　(Fc)　and　secondary　antibody　(Ab(2))　on　the　surface　of　nanogold　(Au)　as　signaling　amplifier　(Ab(2)-Au-Fc),　a　simple　and　sensitive　sandwich-type　electrochemical　immunoassay　(STEM)　of　Tg　was　designed　herein　for　the　first　time.　In　brief,　CNTs　show　large　surface　area　and　conductivity,　while　CD　offers　superior　host-guest　recognition　capability　that　can　bound　with　Ab(1);　meanwhile,　Fc　probe　can　offer　stable　electrochemical　signal　that　is　proportionable　to　the　concentration　of　Tg.　Under　the　optimum　conditions,　the　proposed　STEM　platform　shows　excellent　sensing　results　for　Tg　detection:　a　considerable　low　analytical　detection　(0.5　ng　mL(-1))　and　wide　linearity　(2　to　200　ng　mL(-1)),　suggesting　the　designed　STEM　platform　offers　potential　real　applications　for　detect　Tg.