Efficient　recovery　of　nitrous　oxide　(N2O)　through　heterotrophic　denitrification　with　the　help　of　Fe(II)EDTA-NO　as　a　chelating　agent　has　been　regarded　as　an　ideal　technology　to　treat　nitric　oxide　(NO)-rich　flue　gas.　In　this　study,　an　in-tegrated　NO-based　biological　denitrification　model　was　developed　to　describe　the　sequential　reduction　of　the　NO　fixed　in　Fe(II)EDTA-NO　with　organic　carbon　as　the　electron　donor.　With　the　inclusion　of　only　the　key　pathways　con-tributing　to　nitrogen　transformation,　the　model　was　firstly　developed　and　then　calibrated/validated　and　evaluated　using　the　data　of　batch　tests　mediated　by　the　identified　functional　heterotrophic　bacteria　at　various　substrates　concen-trations　and　then　used　to　explore　the　possibility　of　enhancing　N2O　recovery　by　altering　the　substrates　condition　and　reactor　setup.　The　results　demonstrated　that　the　optimal　COD/N　ratio　decreased　consistently　from　1.5　g-COD/g-N　at　the　initial　NO　concentration　of　40　g-N/m3　to　1.0　g-COD/g-N　at　the　initial　NO　concentration　of　420　g-N/m3.　Further-more,　sufficiently　increasing　the　headspace　volume　of　the　reactor　was　considered　an　ideal　strategy　to　obtain　ideal　N2O　production　of　86.6　%　under　the　studied　conditions.　The　production　of　high-purity　N2O　(98　%)　confirmed　the　prac-tical　application　potential　of　this　integrated　treatment　technology　to　recover　a　valuable　energy　resource　from　NO-rich　flue　gas.