Lindera　aggregata　(Sims)　Kosterm.　is　a　traditional　Chinese　herb,　which　has　been　proven　to　have　excellent　antibacterial　activity.　In　this　work,　we　firstly　extracted　the　polysaccharides　from　the　leaves　of　Lindera　aggregata　(Sims)　Kosterm.　(LLPs),　and　explored　their　antibacterial　activity　and　related　mechanisms.　The　experimental　results　show　that　LLPs　are　a　good　antibacterial　agent,　which　can　damage　the　cell　structure　of　bacteria　and　lead　to　the　leakage　of　intracellular　lysates.　Compared　with　Escherichia　coli　(E.　coli),　LLPs　showed　better　inhibitory　activity　against　Staphylococcus　aureus　(S.　aureus).　Furthermore,　the　administration　of　LLPs　not　only　led　to　the　upregulation　of　the　levels　of　fructose-1,6-bisphosphate　(F-1,6-P)　and　citric　acid　in　the　glycolysis　and　tricarboxylic　acid　cycle　pathways　in　bacteria,　but　also　resulted　in　the　down-regulation　of　the　levels　of　oxaloacetate　(OAA)　and　1,3-diphosphoglycerate　(1,3-BPG).　This　study　confirmed　that　LLPs　have　good　antibacterial　activity,　and　broaden　the　application　of　the　leaves　of　Lindera　aggregata　(Sims)　Kosterm.　in　the　antibacterial　field.　It　provides　ideas　for　exploring　the　antibacterial　mechanism　of　active　ingredients　of　Chinese　herbal　medicine　through　metabolomics.　©　2022