Owing　to　the　limited　literature　on　the　impact　performance　of　steel　beams　with　hexagonal　web　opening　(SBHWOs),　this　study　conducted　drop　hammer　tests　and　used　finite　element　methods　to　investigate　the　dynamic　response　of　SBHWOs　subjected　to　impact　loads.　The　impact　force,　displacements　and　accelerations　of　the　beam,　and　the　strains　around　a　hexagonal　web　opening　were　collected　during　the　tests.　The　impact　process　was　captured　by　a　high-speed　camera.　Furthermore,　a　finite　element　model　was　developed　to　analyze　the　post-impact　internal　forces　of　SBHWO,　development　and　distribution　of　stresses　in　its　web,　the　effect　of　impact　position,　and　differences　with　solid　webbed　steel　beam　(SWSB).　Results　showed　that　the　impact　force,　displacements,　and　web-post　buckling　of　the　SBHWO　were　significantly　affected　by　impact　energy,　spacing　between　openings,　and　opening　height.　The　average　energy　absorption　ratio　of　the　SBHWOs　was　found　to　be　51.56%.　Additionally,　the　midspan　acceleration　decayed　rapidly　after　impact　and　spread　from　the　midspan　towards　the　supports.　The　finite　element　analysis　proved　that　numerical　models　can　be　used　to　accurately　predict　the　dynamic　responses　of　a　SBHWO　subjected　to　impact　loads.　The　axial　forces,　shear　forces,　and　bending　moments　of　the　SBHWO　redistribute　themselves　during　the　impact,　and　the　magnitude　of　the　internal　forces　increased　very　rapidly　before　decaying　gradually.　The　pattern　through　which　stress　developed　in　the　SBHWO　web　was　obtained　using　finite　element　modeling.　A　position　close　to　the　support　approximately　1/4　of　the　calculated　length　of　the　SBHWO　was　the　most　unfavorable　impact　position.　The　SWSB　was　more　resistant　to　impact　load　than　the　SBHWO.