To　study　the　effect　of　okra　aqueous　extract　on　the　intestinal　flora　of　diabetic　rats.　Taking　normal　SD　rats　as　CK　group,　diabetic　rats　induced　by　40　mg/(kg.bw)　streptozotocin　(STZ)　intraperitoneally　as　M　group,　normal　and　diabetic　rats　gavaged　with　500　mg/(kg.bw)　okra　aqueous　extract　were　CKO　and　MO　groups,respectively.　The　bacterial　flora　of　the　cecum　contents　of　rats　was　analyzed　with　16S　rRNA　V3-V4　sequencing　after　8　weeks　of　the　experiment.　Compared　with　CK,　M　increased　Firmicutes　14.00%　and　decreased　Proteobacteria　19.32%　(P　＜0.01).　Compared　with　M,　MO　down-regulated　Lachnospiraceae　2.64%　(such　as　Blautia,　Anaerostipes),　up-regulated　Peptostreptococcaceae　1.20%　in　Firmicutes,　and　down-regulated　Desulfovibrioceae　3.78%　(such　as　Desulfovibrio)　and　up-regulated　Burkholderia　5.24%　(such　as　Parasutterella)　in　Proteobacteria.　It　is　worth　noting　that　MO　up-regulated　Christensenellaceae　(LDA＞4),　which　was　negatively　correlated　with　body　mass　index,　inflammation,　and　metabolic　syndrome.　The　okra　aqueous　extract　reverses　the　changes　of　the　intestinal　flora　of　STZ-induced　diabetic　rats,　which　decreased　Firmicutes　and　increased　Proteobacteria　by　down-regulating　Lachnospiraceae　and　Desulfovibrionaceae,　up-regulating　Burkholderiaceae,　Christensenellaceae.　The　okra　aqueous　extract　has　the　potential　as　a　functional　food　with　regulating　intestinal　microecology　balance　of　diabetes.