Four　Pseudomonas　putida　strains　isolated　from　spoiled　tilapia　were　divided　into　three　adhesion　abilities-high,　medium,　and　low-by　an　in　vitro　mucus　model.　Four　strains　had　no　significant　difference　in　spoilage　ability　to　the　inoculated　fish　fillets.　However,　according　to　the　in　vivo　experiment,　the　spoilage　caused　by　the　four　P.putida　was　positively　correlated　with　their　adhesion　abilities.　High　adhesion　strains　not　only　caused　more　TVB-N　in　chilled　fish,　but　also　activated　the　spoilage　activity　of　intestinal　flora.　The　diversity　of　intestinal　flora　and　the　changes　in　volatile　components　in　fish　were　detected　by　high-throughput　sequencing　and　SPME-GC/MS.　The　strains　with　high　adhesion　abilities　significantly　changed　the　intestinal　flora,　which　led　to　a　significant　increase　in　low-grade　aldehydes,　indole,　and　esters　in　flesh　of　fish,　as　well　as　the　production　of　a　fishy　and　pungent　odor.　The　intestinal　adhesion　ability　of　spoilage　bacteria　was　considered　the　key　factor　in　spoilage　of　chilled　fish.