Nanomaterials　with　enzyme-like　characteristics　(called　nanozymes)　show　their　extreme　potentials　as　alternatives　to　natural　enzymes.　Covalent　organic　frameworks　(COFs)　as　metal-free　nanozymes　have　attracted　huge　attention　for　catalytic　applications　due　to　their　flexible　molecular　design　and　synthetic　strategies　and　conjugated,　porous,　and　chemically　stable　architectures.　Designing　high-performance　two-dimensional　(2D)　porous　COF　materials　embedded　with　functional　building　units　for　modulating　nanozymes＇　catalytic　activity　is　of　immense　importance　in　contemporary　research.　The　proper　combination　of　donor-acceptor　(D-A)　fragments　within　a　porous　COF　skeleton　is　an　effective　strategy　to　decrease　the　band　gap　and　provide　a　strong　charge-transfer　pathway　for　highly　effective　charge　separation.　Herein,　two　donor-acceptor　heteroporous　COFs　using　an　electron-deficient　4,4＇-(thiazolo[5,4-d]thiazole-2,5-diyl)dibenzaldehyde　(Tz)　unit　or　4,4＇-(benzo[c][1,2,5]thiadiazole-4,7-diyl)dibenzaldehyde　(Td)　unit　and　electron-rich　tetrakis(4-aminophenyl)ethane　(ETTA)　linkers　were　presented.　The　resulting　crystalline　and　heteroporous　COFs　showed　outstanding　oxidase-like　activity　under　light　irradiation,　which　can　catalyze　the　oxidation　of　typical　substrates　and　corresponding　evolution　in　color　and　absorption.　The　light-activatable　ETTA-Tz　COF　with　prominent　oxidase-like　activity　can　serve　as　a　colorimetric　probe　for　quantitative　detection　of　sulfide　ions　with　a　linear　range　of　1-50　mu　M　and　a　detection　limit　of　0.27　mu　M　within　3　min.　The　colorimetric　approach　could　also　be　used　for　sulfide　ion　detection　in　human　serum　samples.　The　research　demonstrated　the　future　potential　of　D-A　motifs　within　fully　conjugated　COFs　to　obtain　excellent　mimic　enzyme　activity.