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学者姓名：林子俺
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Abstract ：
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has become a robust tool for analyzing a variety of biomacromolecules. However, the strong background interference produced by conventional organic matrices hinders the detection of small molecule analytes, which restricts the widespread application of MALDI-MS in metabolomics studies. Consequently, developing new organic matrices is urgently needed to overcome these issues. In this study, 1,4-Dioxo-1,2,3,4-tetrahydrophthalazine-6-carboxylic acid (DTCA) was firstly employed as a new matrix for MALDI-MS to enhance the detection of low molecular weight compounds because of its strong UV absorption, less matrix background interference, high ionization efficiency for metabolites, and good reproducibility. Considering these advantages, DTCA was used to analyze endogenous metabolites in the serum samples of imidacloprid (IMI)-exposed mice via MALDI-MS in positive ion mode. By combining with machine learning, the differentiation between imidacloprid-exposed mice and control mice was successfully achieved, and 39 metabolites were estimated as potential biomarkers. Additionally, potentially disrupted metabolic pathways were revealed. These results indicate that DTCA, as a new and powerful matrix in positive ion mode, has great potential for applications in the detection of small molecules.
Keyword ：
1, 4-Dioxo-1, 2, 3, 4-tetrahydrophthalazine-6-carboxylic acid (DTCA) 1, 4-Dioxo-1, 2, 3, 4-tetrahydrophthalazine-6-carboxylic acid (DTCA) 4-tetrahydrophthalazine-6-carboxylic acid (DTCA) 4-tetrahydrophthalazine-6-carboxylic acid (DTCA) MALDI-MS MALDI-MS Metabolic analysis Metabolic analysis
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| GB/T 7714 | Yan, Jingjing , Xie, Wen , Jin, Yingxue et al. 1,4-Dioxo-1,2,3,4-tetrahydrophthalazine-6-carboxylic acid as a novel MALDI matrix for enhanced analysis of metabolites induced by imidacloprid exposure [J]. | TALANTA , 2025 , 286 . |
| MLA | Yan, Jingjing et al. "1,4-Dioxo-1,2,3,4-tetrahydrophthalazine-6-carboxylic acid as a novel MALDI matrix for enhanced analysis of metabolites induced by imidacloprid exposure" . | TALANTA 286 (2025) . |
| APA | Yan, Jingjing , Xie, Wen , Jin, Yingxue , Cai, Zongwei , Lin, Zian . 1,4-Dioxo-1,2,3,4-tetrahydrophthalazine-6-carboxylic acid as a novel MALDI matrix for enhanced analysis of metabolites induced by imidacloprid exposure . | TALANTA , 2025 , 286 . |
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RationalPeople are widely exposed to parabens in their daily life, but parabens are endocrine disrupting chemicals that pose a threat to human health. Therefore, establishing a rapid screening method to enhance monitoring of parabens is necessary. Herein, a covalent organic framework (COF) nanofilm-assisted laser desorption ionization mass spectrometry (LDI-MS) method was established to screen parabens in personal care products (PCPs).MethodsTAPB-TFPB-COF nanofilm was synthesized on indium tin oxide (ITO) glass and used as LDI-MS substrates. To observe the practicability of TAPB-TFPB-COF nanofilm-assisted LDI-MS, the results of this method for analyzing small molecules such as parabens, estrogens, and bisphenols were compared with those of the conventional organic matrix 9-aminoacridine (9-AA), and the reproducibility and detection limit were further verified. Finally, the method was applied to screen parabens in PCPs.ResultsTAPB-TFPB-COF nanofilm-assisted LDI-MS analyzed small molecules such as parabens, estrogens, and bisphenols with higher mass spectral signals and cleaner mass spectral backgrounds compared with 9-AA. Meanwhile, the method analyzed methylparaben (MeP) with high reproducibility (RSD = 6.96%) and low detection limit (1.64 mu M) and performed well for rapid screening of parabens in PCPs.ConclusionTAPB-TFPB-COF nanofilm-assisted LDI-MS for analyzing small molecules such as parabens, estrogens, and amino acids offered the advantages of rapid analysis, a clean background, and good reproducibility. The method was successfully applied to detecting parabens in PCPs, demonstrating the practical utility of LDI-MS based on TAPB-TFPB-COF nanofilm for analyzing parabens in complex samples.
Keyword ：
covalent organic framework nanofilms covalent organic framework nanofilms endocrine disrupting chemicals endocrine disrupting chemicals laser desorption ionization mass spectrometry laser desorption ionization mass spectrometry parabens parabens personal care products personal care products
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| GB/T 7714 | Zheng, Wenjun , Yan, Jingjing , Jin, Yingxue et al. Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for Rapid Screening of Parabens in Personal Care Products [J]. | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2025 , 39 (7) . |
| MLA | Zheng, Wenjun et al. "Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for Rapid Screening of Parabens in Personal Care Products" . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY 39 . 7 (2025) . |
| APA | Zheng, Wenjun , Yan, Jingjing , Jin, Yingxue , Lin, Zian , Cai, Zongwei . Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for Rapid Screening of Parabens in Personal Care Products . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2025 , 39 (7) . |
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Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has been regarded as one of the prominent techniques in small molecule analysis, which employs nanostructured materials as substrates to replace conventional organic matrices for laser absorption and analyte desorption/ionization. The rational design and development of nanostructured substrates, particularly metal-organic frameworks (MOFs) and covalent-organic frameworks (COFs), are crucial for enhancing SALDI-MS performance. This review aims to cover the latest developments (within 5 years) in nanostructured substrates in SALDI-MS, including carbon- and silicon-based materials, metals and their oxide materials, MOFs, COFs, and other materials. The key design parameters that affect the SALDI-MS efficiency of these nanomaterials in small molecule analysis, such as pore size, surface area, and functional groups, are also discussed. Furthermore, the applications of SALDI-MS in various fields are reviewed. Lastly, the challenges and prospects for future research on the design of nano- structured substrates for SALDI-MS in small molecule analysis are discussed.
Keyword ：
Applications Applications mass spectrometry mass spectrometry Nanomaterials Nanomaterials Small molecule Small molecule Surface-assisted laser desorption/ionization Surface-assisted laser desorption/ionization
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| GB/T 7714 | Jin, Yingxue , Yan, Jingjing , Cai, Zongwei et al. Advances in nanomaterials for surface-assisted laser desorption ionization mass spectrometry: Applications in small molecule analysis over the past five years [J]. | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2025 , 183 . |
| MLA | Jin, Yingxue et al. "Advances in nanomaterials for surface-assisted laser desorption ionization mass spectrometry: Applications in small molecule analysis over the past five years" . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY 183 (2025) . |
| APA | Jin, Yingxue , Yan, Jingjing , Cai, Zongwei , Lin, Zian . Advances in nanomaterials for surface-assisted laser desorption ionization mass spectrometry: Applications in small molecule analysis over the past five years . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2025 , 183 . |
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RationalBenzophenone derivatives, commonly used as UV filters in personal care products (PCPs), are widely prevalent and raise concerns due to their endocrine-disrupting effects. Sensitive and efficient analytical methods are in demand for their detection. In this study, we developed a TAPB-DMTP-covalent organic framework (COF) nanofilm-assisted laser desorption ionization mass spectrometry (LDI-MS) method for the quantitative analysis of 2,4-dihydroxybenzophenone (BP-1) in PCPs.MethodsThe TAPB-DMTP-COF nanofilm was synthesized on indium tin oxide (ITO) glass and utilized as an LDI-MS substrate. The performance of TAPB-DMTP-COF nanofilm-assisted LDI-MS for analyzing small molecules (e.g., benzophenone derivatives, phthalates, amino acids, sugars, and nucleosides) was compared to conventional organic matrices (alpha-cyano-4-hydroxycinnamic acid [CHCA], 2,5-dihydroxybenzoic acid [DHB], and sinapinic acid [SA]). The reproducibility, salt resistance, sensitivity, and stability of the method were further evaluated. Finally, the technique was applied to quantify BP-1 in PCPs.ResultsThe TAPB-DMTP-COF nanofilm-assisted LDI-MS provided stronger mass spectral signals and cleaner backgrounds for small molecules compared to CHCA, DHB, and SA. The method exhibited high reproducibility (RSD = 6.10%) and stability for up to 30 days. BP-1 in PCPs was quantified with excellent linearity (1-20 mu g/mL, r = 0.9993), a low detection limit (0.3 mu g/mL), and recovery rates of 94.2%-104.4%, demonstrating the potential of TAPB-DMTP-COF nanofilm for sensitive and reliable small-molecule analysis.ConclusionTAPB-DMTP-COF nanofilm-assisted LDI-MS offered the advantages of rapid analysis, clean backgrounds, and reproducibility for detecting small molecules, including benzophenone derivatives. This method successfully quantified BP-1 in PCPs, highlighting its suitability for analyzing complex samples.
Keyword ：
benzophenone derivatives benzophenone derivatives covalent organic framework nanofilm covalent organic framework nanofilm endocrine disruptors endocrine disruptors laser desorption ionization mass spectrometry laser desorption ionization mass spectrometry personal care products personal care products
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| GB/T 7714 | Yan, Ling , Zheng, Wenjun , Lin, Zian . Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for the Determination of Benzophenone Derivatives in Personal Care Products [J]. | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2025 , 39 (10) . |
| MLA | Yan, Ling et al. "Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for the Determination of Benzophenone Derivatives in Personal Care Products" . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY 39 . 10 (2025) . |
| APA | Yan, Ling , Zheng, Wenjun , Lin, Zian . Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for the Determination of Benzophenone Derivatives in Personal Care Products . | RAPID COMMUNICATIONS IN MASS SPECTROMETRY , 2025 , 39 (10) . |
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Membrane technology, as an efficient and eco-friendly separation means, has demonstrated substantial superiority in liquid-based separation and plays a pivotal role in environmental conservation and resource utilization. Traditional polymer-based membranes have long dominated the market for their simple preparation and low cost. But the disordered structure and irregular aperture of conventional amorphous membranes seriously hinder their performance. Recently, the emergence of covalent organic frameworks (COFs) has brought new vigor to membrane-based separation. COFs, connected by covalent bonds, have good crystallization and regular and uniform pore structure, thereby showcasing excellent stability and separation performance, becoming an ideal membrane material. In this paper, the synthesis and modification of COF-based membranes were reviewed, their inherent properties were also introduced. Besides, their applications in liquid-based separation were elaborated. Lastly, the challenges and focal points were discussed, aiming at offering inspirations for the development of COF-based membranes.
Keyword ：
Covalent organic frameworks Covalent organic frameworks Membrane Membrane Separation Separation Synthesis Synthesis Water treatment Water treatment
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| GB/T 7714 | Dong, Shaofeng , Xue, Yuandi , Li, Songtao et al. Recent advances in the synthesis of covalent organic framework-based membranes and their applications for liquid-based separation [J]. | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2025 , 185 . |
| MLA | Dong, Shaofeng et al. "Recent advances in the synthesis of covalent organic framework-based membranes and their applications for liquid-based separation" . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY 185 (2025) . |
| APA | Dong, Shaofeng , Xue, Yuandi , Li, Songtao , Zhang, Yajing , Wang, Shuyi , Lin, Zian . Recent advances in the synthesis of covalent organic framework-based membranes and their applications for liquid-based separation . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2025 , 185 . |
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Bile acids, a representative diagnostic indicator of liver function, are used to visualize the extent of liver injury. Numerous studies have shown that triclosan (TCS) exposure leads to abnormal bile acid metabolism. As a result, there is a requirement to develop a fast and smart means to quantitatively monitor abnormal bile acids from exposure to triclosan in bio-sample. In this work, solid-phase microextraction (SPME) probes of sea urchin-like covalent organic frameworks (COF) were in situ synthesized on steel needles by using 1,3,5-tris(4-aminophenyl) benzene (TAPB) and 2,5-dimethoxybenzene-1,4-dicarboxaldehyde (DMTP) as two organic units and employed for extraction of bile acids. This TAPB-DMTP-COF-SPME possessed an excellent specified surface area (3351 m(2) g(-1)) and a high regular porosity (similar to 3.6 nm), which was an ideal adsorbent to concentrate bile acids efficiently. The created probe, together with electrospray ionization mass spectrometry (ESI/MS), proved to be a fast and specific assay for the detection of bile acids in bio-samples. The proposed method had a low limitation of detection (0.03 mu g L-1), good linearity (R-2 >= 0.9931), wide linear range (0.10-1000.00 mu g L-1) and excellent enrichment factor (63.60-252.00). Based on these excellent properties, it was successful application for the analyzing of bile acids in mice liver and feces, demonstrating the great potential of TAPB-DMTP-COF-SPME-ESI/MS in bile acids detection and liver injury diagnosis.
Keyword ：
Bile acids Bile acids Covalent organic frameworks Covalent organic frameworks Electrospray ionization mass spectrometry Electrospray ionization mass spectrometry Solid-phase microextraction Solid-phase microextraction Triclosan exposure Triclosan exposure
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| GB/T 7714 | Shi, Xinye , Xue, Yuandi , Tu, Yuxin et al. Covalent organic framework-based solid phase microextraction coupled with electrospray ionization mass spectrometry for the quantitative assessment of abnormal bile acids by triclosan exposure in mice [J]. | TALANTA , 2025 , 285 . |
| MLA | Shi, Xinye et al. "Covalent organic framework-based solid phase microextraction coupled with electrospray ionization mass spectrometry for the quantitative assessment of abnormal bile acids by triclosan exposure in mice" . | TALANTA 285 (2025) . |
| APA | Shi, Xinye , Xue, Yuandi , Tu, Yuxin , Chen, Canrong , Zhang, Yajing , Lin, Zian et al. Covalent organic framework-based solid phase microextraction coupled with electrospray ionization mass spectrometry for the quantitative assessment of abnormal bile acids by triclosan exposure in mice . | TALANTA , 2025 , 285 . |
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Nanozymes-based optical sensors are widely used in the field of disease diagnosis, but most of the metal-based nanomaterials with peroxidase-like would not only require the addition of unstable and slightly toxic hydrogen peroxide but also often be affected by their own oxidase-like activity leading to the problems such as poor reproducibility and environmental pollution. Moreover, single-mode biosensors are subject to signal instability, which can lead to false-positive or false-negative results. Hence, we synthesized two three-component covalent organic frameworks (COFs) with the oxidase-like and ascorbate oxidase-like activities using benzo[1,2-b:3,4b':5,6-b"]trithiophene-2,5,8-tricarboxaldehyde (BTT) and 1,3,5-tris(4-formylphenyl)benzene (TFPB) as the nodes, and 1,4-diaminobenzene (PA) and 4,4-diaminodiphenyl (BD) as the linker units. Both three-component COFs had narrow band gaps (2.16 eV and 2.27 eV), excellent electron transport properties, and high enzymelike specific activities (4.32 U mg(-1) and 1.82 U mg(-1)). Based on these, a colorimetric and ratiometric fluorescence dual-mode cascade catalytic platform was developed to detect the activity of alkaline phosphatase (ALP), a marker for diseases such as liver and bone. The constructed analytical methods had a detection limit as low as 0.10 U L-1 and were successfully used to detect ALP levels in human serum. This work also provides a new idea for developing an efficient multimodal detection and evaluation platform using nanozymes based on novel COFs.
Keyword ：
Alkaline phosphatase Alkaline phosphatase Biosensor Biosensor Dual-mode detection Dual-mode detection Light-activated nanozymes Light-activated nanozymes Three-component covalent organic frameworks Three-component covalent organic frameworks
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| GB/T 7714 | Hu, Cong , Li, Guorong , Xue, Yuandi et al. Light-activated bifunctional nanozymes based on three-component covalent organic frameworks for colorimetric and ratiometric fluorescence dual-mode detection of alkaline phosphatase [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 422 . |
| MLA | Hu, Cong et al. "Light-activated bifunctional nanozymes based on three-component covalent organic frameworks for colorimetric and ratiometric fluorescence dual-mode detection of alkaline phosphatase" . | SENSORS AND ACTUATORS B-CHEMICAL 422 (2025) . |
| APA | Hu, Cong , Li, Guorong , Xue, Yuandi , Liu, Jin , Cai, Zongwei , Lin, Zian . Light-activated bifunctional nanozymes based on three-component covalent organic frameworks for colorimetric and ratiometric fluorescence dual-mode detection of alkaline phosphatase . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 422 . |
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随着工业的发展,重金属及放射性物质的污染加剧,同时对稀有金属的需求上升,因此对金属进行分离富集非常必要.共价有机框架材料(COFs)具有比表面积大、孔隙率高、结晶度高和孔径可调等特点,在金属分离富集领域展现出巨大潜力.通过结合特定官能团,能够提高COFs对某些金属离子的选择性,COFs材料稳定的共价键连接方式也增强了其在恶劣环境中的稳定性.鉴于此,该文对近年来COFs在金属分离富集中的应用进行了综述,重点对COFs的不同连接方式,及其在贵金属、重金属、稀土元素和放射性元素中的分离富集以及吸附机理进行介绍.最后对COFs在金属分离富集中存在的挑战进行分析,并对未来的研究趋势进行了展望.
Keyword ：
共价有机框架 共价有机框架 分离 分离 富集 富集 金属 金属
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| GB/T 7714 | 李松涛 , 董少锋 , 林月琳 et al. 共价有机框架材料在金属分离富集中的应用 [J]. | 分析测试学报 , 2025 , 44 (1) : 51-60 . |
| MLA | 李松涛 et al. "共价有机框架材料在金属分离富集中的应用" . | 分析测试学报 44 . 1 (2025) : 51-60 . |
| APA | 李松涛 , 董少锋 , 林月琳 , 孙颖 , 林子俺 . 共价有机框架材料在金属分离富集中的应用 . | 分析测试学报 , 2025 , 44 (1) , 51-60 . |
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Triphenyl phosphate (TPP), a wide-used organophosphate flame retardants (OPFRs), is suspected to be a risk factor for the female-specific cancers, but underlying toxicity mechanisms of environmentally relevant dose exposure remain unclear. Herein, a strategy of spherical covalent organic framework (TPB-BPTP-COF)-assisted laser desorption ionization mass spectrometry (LDI-MS), which benefited from fast analysis speed, facile sample preprocessing, and high throughput, was proposed for unveiling the biomarkers of breast cancer (BC) and the relationship between TPP exposure and progression of BC in mice by serum metabolism analysis. The results displayed that 13 metabolites associated with BC development were up-regulated in experimental group versus healthy control mice. Moreover, long-term exposure to environmentally relevant doses of TPP was found to promote BC, mainly by affecting glycolysis/gluconeogenesis, pyrimidine metabolism, pantothenic acid and CoA biosynthesis, and β-alanine metabolism. This work proved the potential application of COFs as LDI-MS substrates in analyzing complex biological samples, and also revealed the risk of long-term low-dose exposure to TPP in the development of BC. © 2024 Elsevier B.V.
Keyword ：
Biomarkers Biomarkers Body fluids Body fluids Ionization potential Ionization potential Metabolites Metabolites
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| GB/T 7714 | Ouyang, Dan , Dan, Akang , Lin, Zian et al. Spherical covalent-organic framework-assisted laser desorption ionization mass spectrometry reveals the promotional effect of triphenyl phosphate on breast cancer in mice [J]. | Science of the Total Environment , 2024 , 955 . |
| MLA | Ouyang, Dan et al. "Spherical covalent-organic framework-assisted laser desorption ionization mass spectrometry reveals the promotional effect of triphenyl phosphate on breast cancer in mice" . | Science of the Total Environment 955 (2024) . |
| APA | Ouyang, Dan , Dan, Akang , Lin, Zian , Cai, Zongwei . Spherical covalent-organic framework-assisted laser desorption ionization mass spectrometry reveals the promotional effect of triphenyl phosphate on breast cancer in mice . | Science of the Total Environment , 2024 , 955 . |
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Targeted construction of new covalent organic frameworks (COFs) with specific purposes and rationalities to build colorimetric assay platform for environmental pollutant monitoring have attracted increasing interest. However, it is still challenging due to lack of available coordination sites inside COFs pores and only a slight bonding ability for anchoring metal. In this work, a two-dimensional (2D) COFs (termed as Tz-COF) with high crystallinity, excellent chemical stability, and abundant sulfur coordination in its skeletons was synthesized and used for the confined growth of Au NPs. It was found that the Au NPs showed significant dispersibility for the support of Tz-COF. The proposed Tz-COF@Au NPs possessed outstanding Hg 2 + -activated peroxidase-like activity benefited from physicochemical properties of gold amalgam and synergistic effect between COFs and Au NPs to oxidize chromogenic substrate. Based on highly efficient activity and distinctive color evolution, the strategy for detecting Hg 2 + was developed and successfully applied to determine the content of Hg 2 + in real environmental samples. This work manifests that a potential strategy to establish a colorimetric assay platform for environmental pollutant monitoring based on the targeted manufacturing of novel COFs with specific functions. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
Keyword ：
Au nanoparticles Au nanoparticles Covalent organic frameworks Covalent organic frameworks Hg2+-activated peroxidase-like activity Hg2+-activated peroxidase-like activity Sulfur coordination Sulfur coordination Thiazole Thiazole
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| GB/T 7714 | Li, Guorong , Wu, Yijing , Zhong, Chao et al. Pre designe d covalent organic framework with sulfur coordination: Anchoring Au nanoparticles for sensitive colorimetric detection of Hg(II) [J]. | CHINESE CHEMICAL LETTERS , 2024 , 35 (5) . |
| MLA | Li, Guorong et al. "Pre designe d covalent organic framework with sulfur coordination: Anchoring Au nanoparticles for sensitive colorimetric detection of Hg(II)" . | CHINESE CHEMICAL LETTERS 35 . 5 (2024) . |
| APA | Li, Guorong , Wu, Yijing , Zhong, Chao , Yang, Yixin , Lin, Zian . Pre designe d covalent organic framework with sulfur coordination: Anchoring Au nanoparticles for sensitive colorimetric detection of Hg(II) . | CHINESE CHEMICAL LETTERS , 2024 , 35 (5) . |
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