Recently　we　have　demonstrated　that　the　surface　plasmon　of　noble　metal　nanoparticles　can　effectively　enhance　the　ECL　intensity　of　Ru(bpy)32+,　and　we　named　this　detection　principle　as　surface-enhanced　electrochemiluminescence　(SEECL-I).　However,　SEECL　based　on　photomultiplier　tube　(PMT)　detection　can　only　detect　one　target　at　a　time,　which　is　not　suitable　for　multiple　targets　detection.　In　this　work,　we　combined　our　previous　developed　SEECL　with　a　bioimaging　device　to　develop　a　novel　multiplexed　immunassay　for　simultaneous　and　fast　analysis　of　cancer　markers.　A　core-shell　nanocomposite　consisted　of　gold-silicon　dioxide　nanoparticles　doped　with　Ru(bpy)32+(Au@SiO2-Ru)　with　strong　ECL　emission　was　employed　as　ECL　label　due　to　the　localized　surface　plasmon　resonance　(LSPR)　of　AuNPs,　which　can　significantly　enhance　the　ECL　emission　of　Ru(bpy)32+.　The　ECL　signals　from　the　4　×　4　electrode　arrays　were　collected　using　the　constant　potential　method　(current-time　curve　method)　imaging　with　a　sCOMS　camera.　As　a　proof-of-concept　application,　we　demonstrated　the　use　of　the　proposed　SEECL-I　for　simultaneous　detection　of　carcinoembryonic　antigen　(CEA),　neuron　specific　enolase　(NSE),　and　squamous　cell　carcinoma　antigen　(SCC)　in　exhaled　breath　condensates　(EBCs)　with　low　detection　limit　(LOD)　of　0.17,　0.33,　and　0.33　pg/mL　(S/N　=　3),　respectively.　The　results　demonstrated　that　the　proposed　SEECL-I　strategy　can　provide　a　high　sensitivity,　fast　analysis,　and　high-throughput　platform　for　clinical　diagnosis　of　cancer　markers　in　EBCs.　©　2022　American　Chemical　Society.