Identification　of　different　mitochondrial　reactive　oxygen　species　(ROS)　simultaneously　in　living　cells　is　vital　for　understanding　the　critical　roles　of　different　ROS　in　biological　processes.　To　date,　it　remains　a　great　challenge　to　develop　ROS　probes　for　direct　and　simultaneous　identification　of　multiple　ROS　with　high　specificity.　Herein,　we　report　a　SERS-borrowing-strategy-based　nanoprobe　(Au@Pt　core-shell　nanoparticles)　for　simultaneous　and　direct　identification　of　different　ROS　by　their　distinct　Raman　fingerprints.　Isotope　substitution　experiments　and　DFT　calculations　confirmed　the　ability　of　Au@Pt　nanoprobe　to　capture　and　identify　different　mitochondrial　ROS　(i.e.　center　dot　OOH,　H2O2,　and　center　dot　OH).　When　functionalized　with　triphenylphosphine　(TPP),　the　Au@Pt-TPP　nanoprobe　located　to　mitochondria　and　detected　multiple　ROS　simultaneously　in　living　cells　under　oxidative　stimulation.　Our　method　offers　a　new　tool　for　the　study　of　the　functions　of　various　ROS　in　biological　processes.