Direct and Simultaneous Identification of Multiple Mitochondrial Reactive Oxygen Species in Living Cells Using a SERS Borrowing Strategy - Details

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Abstract：

Identification　of　different　mitochondrial　reactive　oxygen　species　(ROS)　simultaneously　in　living　cells　is　vital　for　understanding　the　critical　roles　of　different　ROS　in　biological　processes.　To　date,　it　remains　a　great　challenge　to　develop　ROS　probes　for　direct　and　simultaneous　identification　of　multiple　ROS　with　high　specificity.　Herein,　we　report　a　SERS-borrowing-strategy-based　nanoprobe　(Au@Pt　core–shell　nanoparticles)　for　simultaneous　and　direct　identification　of　different　ROS　by　their　distinct　Raman　fingerprints.　Isotope　substitution　experiments　and　DFT　calculations　confirmed　the　ability　of　Au@Pt　nanoprobe　to　capture　and　identify　different　mitochondrial　ROS　(i.e.　⋅OOH,　H2O2,　and　⋅OH).　When　functionalized　with　triphenylphosphine　(TPP),　the　Au@Pt-TPP　nanoprobe　located　to　mitochondria　and　detected　multiple　ROS　simultaneously　in　living　cells　under　oxidative　stimulation.　Our　method　offers　a　new　tool　for　the　study　of　the　functions　of　various　ROS　in　biological　processes.　©　2022　Wiley-VCH　GmbH.

Keyword：

Core–Shell Nanoprobe; Living Cells; Reactive Oxygen Species; SERS Borrowing; Spectroscopic Identification

Community：

[ 1 ] [Lin, S.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 2 ] [Ze, H.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 3 ] [Zhang, X.-G.]Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, China
[ 4 ] [Zhang, Y.-J.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 5 ] [Song, J.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 6 ] [Zhang, H.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 7 ] [Zhong, H.-L.]College of Chemistry, Fuzhou University, Fuzhou, 350108, China
[ 8 ] [Yang, Z.-L.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 9 ] [Yang, C.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 10 ] [Li, J.-F.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China
[ 11 ] [Zhu, Z.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, College of Energy, Xiamen University, Xiamen, 361005, China

Reprint 's Address：

[Zhu, Z.]MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, China

Email：

zhuzhi@xmu.edu.cn

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Source ：

Angewandte Chemie - International Edition

ISSN： 1433-7851

Year： 2022

Issue： 25

Volume： 61

1 6 . 6

JCR@2022

1 6 . 1 0 0

JCR@2023

ESI HC Threshold：74

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

30 Days PV： 0

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