The　lateral　ventricle　puncture　is　an　important　step　of　external　ventricular　drain.　It　is　one　of　the　most　basic　but　challenging　skills　that　must　be　mastered　by　physicians.　For　improving　the　lateral　ventricle　puncture　skill,　a　novel　virtual　reality　simulation　training　system　equipped　with　haptic　feedback　was　developed　in　this　paper.　A　series　of　experiments　and　questionnaires　were　conducted　to　evaluate　the　fidelity　of　simulated　haptic　force　and　the　effectiveness　of　this　system.　Both　the　forces　generated　by　the　haptic　device　during　the　virtual　puncture　and　that　generated　during　puncturing　on　a　pig　brain　were　obtained　and　compared.　The　results　indicate　that　these　two　forces　have　the　similar　varying　tendency　under　different　puncturing　conditions.　In　addition,　two　groups　of　neurosurgical　interns　named　A　(trained　without　this　system)　and　B　(trained　with　this　system)　were　selected　to　verify　the　effectiveness　of　this　system.　The　operation　metrics,　including　operative　dictation,　operation　time,　positioning　of　Kocher’s　point,　times　of　repeated　punctures,　and　the　punctured　position　on　lateral　ventricle,　were　assessed　by　the　chief　physician　for　both　groups.　The　results　show　that　group　B　achieved　higher　scores　than　group　A　in　all　operation　metrics　except　only　operative　dictation　(P　=　0.001).　This　indicates　that　the　proposed　virtual　training　system　is　an　effective　aid　in　training　neurosurgery　physicians’　lateral　ventricle　puncture　skill.　©　2021,　The　Author(s),　under　exclusive　licence　to　Springer-Verlag　London　Ltd.,　part　of　Springer　Nature.