The　polycaprolactone　(PCL)　and　nano-hydroxyapatite　(nHAP)　composite　is　an　attractive　material　for　bone　scaffolds　with　excellent　mechanical　properties　and　osteoinductivity.　It　also　exhibited　good　biocompatibility　as　well　as　controllable　biodegradability.　We　have　recently　developed　a　PCL　and　nHAP　composite　scaffold,　which　is　embedded　with　poly　(lactic-co-glycolic　acid)　(PLGA)　microspheres　(mu　s).　It　achieved　controlled　delivery　of　bioactive　factors.　In　this　study,　Icariin　(ICA)　encapsulated　with　PLGA　mu　s　was　embedded　in　3D　printed　PCL/nHAP　scaffolds　to　facilitate　in　situ　bone　regeneration.　The　scaffold　exhibited　excellent　mechanical　performance　owing　to　the　nHAP.　The　PCL/nHAP　scaffold　showed　sustainable　release　of　ICA　as　the　PCL　degraded.　The　PCL　degradation　produced　cracks　on　the　surface　of　the　scaffold,　and　then　the　PLGA　mu　s　was　exposed　to　phosphate　buffer　solution.　The　released　ICA　promoted　the　osteogenic　differentiation　of　MC3T3-E1.　Consistently,　in　vivo　studies　showed　that　the　composite　scaffolds　releasing　ICA　promoted　the　healing　of　calvaria　bone.　In　conclusion,　PCL/　PLGA　mu　s/nHAP　composite　scaffold　by　3D　printing　may　serve　as　an　efficient　material　for　bone　tissue　repair　and　regeneration.