A　series　of　cyclometalated　iridium　(III)　complexes,　[(dfppy)(2)Ir(bpy-sugar)]Cl　(1),　[(tpy-COOH)(2)Ir(bpy-sugar)]　Cl　(2)　and　[(mpbq)(2)Ir(bpy.　sugar)]Cl　(3)　(dfppy=2-(2,4-difluorophenyl)pyridine,　tpy-COOH=4-(2＇-pyridyl)benzoic　acid,　mpbq=2-methyl-3-phenylbenzo[g]quinoxaline,　bpy-sugar=4,4＇-bis(1-thio-beta-D-glucosemethyl)-2,2＇-bipyridine),　were　designed　and　synthesized　with　cyclometalated　C＜^＞N　ligand　and　bpy-sugar　assistant　ligands.　Their　structures　were　characterized　by　NMR,　MS,　IR　spectroscopy　and　elemental　analysis.　Their　photophysical　properties　and　applications　in　cell　imaging　were　studied.　Through　the　regulation　of　auxiliary　cyclometalated　ligands,　the　photoluminescence　of　the　complexes　can　be　tuned　from　yellow　to　near.　infrared　light.　In　aqueous　solution,　complexes　1　and　2　displayed　yellow　emissions　with　the　maximum　wavelengths　at　about　546　and　584　nm,　respectively.　While　complex　3　displayed　near　infrared　emission　with　wavelength　at　about　780　nm　in　a　mixed　solution　of　H2O　and　dimethyl　sulfoxide　(39:1,　V/V).　The　quantum　efficiencies　of　complexes　1　and　2　were　16.9%　and　3.1%,　respectively.　The　quantum　yield　of　complex　1　was　significantly　higher　than　that　of　ruthenium　(II)　tris(2,2＇-bipyridine).　The　photoluminescence　of　complex　3　was　weak,　and　the　integrating　sphere　cannot　obtain　accurate　quantum　yield.　However,　its　photoluminescence　in　the　near.　infrared　region　was　expected　to　be　used　as　a　near-infrared　probe　for　biological　analysis.　The　lifetimes　of　complexes　1　and　2　were　0.22　and　0.10　mu　s,　respectively.　Modifying　different　groups　on　the　ligand　can　increase　the　cell　penetration　and　water　solubility　of　the　complexes,　and　realize　the　imaging　analysis　of　the　complex　in　the　cell.　The　iridium　(III)　complexes　could　penetrate　the　cell　membrane　and　enter　the　cell,　and　overlap　with　the　part　of　the　nucleus　stained　by　4,6.bialamidine-2-phenylindole　(DAPI),　indicating　that　it　had　entered　the　nucleus.　The　photoluminescence　of　the　iridium　(III)　complexes　was　good　in　living　cells,　showing　that　the　iridium　(III)　complexes　was　still　stable　in　the　biological　environment.