Beyond　extraordinary　in　vivo　accurate　gene　editing　and　regulation　capabilities,　the　CRISPR-Cas-associated　biotechnology　has　created　a　new　era　of　in　vitro　nucleic　acid　sensing　due　to　its　inherent　high-efficiency　enzyme　cleavage　activity　and　robustness.　However,　most　of　the　existing　CRISPR-Cas　systems　are　largely　involve　fluorescent　reporters　or　lateral　flow　strips　biosensor　and　can＇t　fully　explore　their　potential　applications　(sensitivity　and　field-deployable)　due　to　the　lack　of　effective　signal　transduction　and　wireless　data　transmission　with　smartphone　readout.　Herein,　a　CRISPR-Cas12a-mediated　instrument-free　based　on　exible　interdigitated　electrodes-modified　piezoresistive　block　[abrasive　paper-molded　microstructure　polydimethylsiloxane　(PDMS)　and　MXene　(Ti3C2Tx)-PEDOT:PSS　film]　was　designed　for　point-of-care　of　human　papillomavirus　(HPV)-related　DNA　with　integrated-module　smartphone　visual　readout.　Biotin/thiol-modification　single-stranded　DNA　(ssDNA),　acts　as　a　linker　between　Au@Pt　nanoparticles　(Au@PtNPs)　and　streptavidin-coated　magnetic　bead　(MB),　are　non-specifically　cleaved　by　Cas12a　when　the　guide　RNA　binds　to　the　target　HPV-DNA.　Released　and　separated　Au@PtNPs　efficiently　catalyzes　H2O2　to　O2,　and　further　compress　the　Ti3C2Tx-PEDOT:PSS/PDMS　in　a　3D-printed　home-made　pressure-tight　vessel,　thus　causing　the　increasing　current　of　the　whole　circuit　thanks　to　contacting　deformation　of　Ti3C2Tx-PEDOT:PSS/PDMS　module　and　interdigital　electrode.　After　integrating　the　Bluetooth　device　and　wireless　sensing　technology　in　the　circuit,　the　current　signal　of　the　target　DNA　can　be　wirelessly　collected　and　recorded　in　real-time　and　further　transmitted/displayed　to　the　mobile　terminal　of　the　smartphone.　This　all-in-one　detection　mode　not　only　bridges　the　technological　gap　between　biological　signal　conduction,　wireless　transmission,　and　smartphone　interface　but　also　improves　the　portability　and　the　sensitivity　(more　than　one　order　of　magnitude　lower　than　that　of　traditional　CRISPR-Cas12a　biosensors).　We　expect　that　a　powerful　CRISPR-Cas12a　system　coupled　with　piezoresistive　sensor　with　wireless　transmission　technology　will　become　a　great　demonstration　and　widely　used　in　real-time　wireless　biomedical　analysis,　portable　point-of-care　health　monitoring,　and　molecular　diagnostics.　©　2020　Elsevier　Ltd