A　hydrogel　is　a　novel　high　molecular　polymer　that　forms　a　three-dimensional　network　structure　by　polymer　chemical　or　physical　cross-linking.　In　this　experimental　study,　a　chitosan-hyaluronic　acid　gel　was　used　as　a　carrier,　and　nano-hydroxyapatite/collagen　was　composited　to　prepare　a　gel-based　composite　for　3D　bio-printing.　The　properties　of　the　composite　gel　were　analyzed　by　in　vitro　gel　test,　rheological　analysis,　infrared　spectroscopy　and　scanning　electron　microscopy.　The　results　showed　that　the　chitosan-hyaluronic　acid　gel　with　different　mineralized　collagen　concentrations　could　be　gelled　rapidly　at　37　°C　for　5　min.　Rheology　experiments　show　that　CS-HA　mineralized　collagen　has　shear　thinning　characteristics　suitable　for　3D　bio-printing.　The　hydrogel　was　observed　to　have　a　cross-linked　morphology　by　scanning　electron　microscopy,　and　the　porous　structure　was　good.　Has　a　high　biomedical　re-research　value　and　use　value.　©　2019,　Springer　Nature　Singapore　Pte　Ltd.