The　application　of　surface-enhanced　Raman　scattering　(SERS)　in　aqueous　sample　detection　is　normally　limited　by　the　low　affinity　between　analytes　and　SERS-active　nanoparticles.　Furthermore,　a　tendency　of　uncontrollable　aggregation　of　solute　(nanoparticles　or　analytes)　can　cause　poor　reproducibility　of　the　detected　SERS　signal.　Herein,　a　ready-to-use　plasmonic　gel　bead　was　developed　for　rapid　and　effective　detection　of　aqueous　samples　with　high　sensitivity　and　reproducibility.　The　SERS　gel　bead　is　made　of　calcium　alginate　gel　beads　(CAGBs)　that　contain　gold　nanobipyramids　(Au　NBPs)　and　an　aqueous　sample,　which　can　be　prepared　and　detected　within　only　1　min.　Au　NBPs/CAGBs　can　generate　an　in-depth　three-dimensional　SERS-active　gel　network　for　trapping　analytes　and　offering　a　uniform　hotspot　region,　which　produces　a　reproducible　and　uniform　signal.　Using　rhodamine　6G　as　a　model　target,　the　proposed　method　exhibits　excellent　reproducibility　with　a　relative　standard　deviation　of　6.57%　and　a　detection　limit　of　0.4　nM.　Then,　Au　NBPs/CAGBs　were　applied　to　quantitatively　detect　serum　uric　acid　in　the　range　of　10-1000　μM　and　a　limit　of　detection　of　0.18　μM,　and　the　results　were　strongly　consistent　with　those　of　the　commercial　ELISA　method.　This　work　offers　a　low-cost　and　easy　route　for　the　fabrication　of　a　versatile　SERS　substrate　for　monitoring　disease-related　biomarkers　and　point-of-care　testing.　©　2021　American　Chemical　Society.