In　order　to　improve　crosslinking　strength　and　hydrogel　stability,　cellulose　ether　was　introduced　into　calcium　alginate　and　crosslinked　to　form　interpenetrating　networks(IPNs).　Protein　and　emulsion　dually　imprinted　calcium　alginate　microspheres　were　prepared　with　the　modified　hydrogel　and　were　applied　for　selective　rebinding.　The　microspheres　were　found　to　be　more　mechanically　stable　and　more　resisting　to　0.9%　sodium　chloride　aqueous　solution,　and　the　damage　ratio　was　reduced　from　24.4%　to　9.84%　according　to　oscillation　test.　The　infrared　spectra　measurement　indicated　that　new　chemical　bonds　were　formed　between　glutaraldehyde　and　cellulose,　which　constructed　the　interpenetrating　networks.　Protein　molecule　imprinting　and　rebinding　experiments　were　performed　and　imprinting　efficiency　was　increased　from　2.19　to　2.70　towards　target　molecular　in　the　microspheres　modified　by　IPNs.　An　improvement　in　separation　factor　from　1.478　to　18.88　was　confirmed　by　ion-exchange　chromatography　experiments.　The　above　results　could　be　ascribed　to　the　compact　network　structure　due　to　the　association　of　stronger　covalent　crosslinkages　in　the　IPNs　and　the　hydrophobic　micro-environment.　The　IPNs　enhance　the　imprint　structure　stability　and　increase　rebinding　specificity.