The　utilization　of　processed　waste　materials　in　road　construction　is　of　utmost　significance　from　the　environmental　and　economic　viewpoints.　In　this　paper,　waste　cooking　oil　(WCO)　and　ground　tire　rubber　(GTR)　were　applied　to　modify　the　asphalt　binder.　Based　on　the　optimal　content　of　both　modifiers,　sixteen　asphalt　samples　with　different　combinations　of　the　two　wastes　were　designed.　Relevant　tests　including　basic　properties　along　with　rheological　behavior　and　chemical　characterization　were　carried　out　on　the　modified　asphalt.　The　results　indicated　that　the　modified　asphalt　with　20　wt　%　GTR　and　2.5　wt　%-5　wt.　%　WCO　exhibited　satisfactory　high　temperature　resistance　and　low　temperature　rheological　properties.　The　Fourier　transform　infrared　spectroscopic　investigation　revealed　that　the　presence　of　GTR　increased　the　concentration　of　styrene　and　butadiene　while　the　addition　of　WCO　introduced　fatty　acids　and　esters　to　modified　asphalt.　Overall,　the　road　performance　studies　demonstrate　that　modified　asphalt　mixture　with　5　wt　%　WCO　and　20　wt　%　GTR　displayed　a　markedly　improved　anti-rutting　properties,　water　stability　and　superior　low-temperature　performance　compared　to　normal　asphalt　mixture.　©　2021　Elsevier　Ltd