Herein,　a　facile　and　practical　aptamer-grafted　ionic　affinity　monolith　with　mixed-mode　mechanism　was　explored　as　a　versatile　platform　for　online　specific　recognition　of　polar　and　non-polar　mycotoxins.　The　mixed-mode　mechanism　including　molecular　affinity　adsorption　(between　aptamers　and　targets),　hydrophilic　interaction　and　ionic　interaction　(between　stationary　phase　and　targets)　were　adopted　and　provided　a　better　flexibility　in　adjusting　separation　selectivity　to　reduce　nonspecific　adsorption　with　respect　to　the　single　mode.　Preparation　and　characterization　of　aptamer-based　affinity　monoliths　were　investigated,　The　characterization　of　pore　size　distribution,　Brunauer-Emmett-Teller　(BET)　surface　area　and　the　specificity　and　cross-reaction　were　also　evaluated.　As　a　result,　the　hydrophilic　nature　and　negative　charge　on　affinity　monolith　were　obtained.　Multiple　interactions　including　aptamer　affinity　binding,　hydrophilic　interaction　(HI)　and　ion　exchange　(IE)　could　be　adopted　for　online　selective　extraction.　Specific　recognitions　of　polar　ochratoxin　A　(OTA),　non-polar　zearalenone　(ZEN)　and　aflatoxin　B1　(AFB1)　was　fulfilled　with　LODs　as　0.03,　0.05　and　0.05　μg/L,　respectively.　Applied　to　real　cereals,　good　recoveries　of　the　fortified　OTA,　AFB1　and　ZEN　were　achieved　as　92.6　±　1.3%　~　95.6　±　1.3%　(n=3),　93.9　±　2.3%　~　98.2　±　3.4%　(n=3)　and　92.7　±　2.0%　~　96.9　±　3.5%　(n=3)　in　corn,　wheat　and　rice,　respectively.　The　results　displayed　that　Apt-MCs　with　hydrophilic　and　ionic　interaction　mixed-mode　mechanism　were　efficient　enough　and　competent　for　the　online　recognition　of　mycotoxins　in　cereals.　©　2021