Rare　electrochemiluminescence　(ECL)　sensors　have　been　developed　based　on　the　direct　regulation　of　ionic　current　because　it　is　difficult　to　establish　a　relationship　between　ionic　current　and　ECL　reporting.　Ionic　current　can　be　adjusted　by　the　effective　radius　and　charge　density　of　a　functionalized　microchannel　and　is　frequently　adopted　to　develop　electrical　sensors.　Here,　we　show　a　novel　ECL　sensing　platform　that　combines　the　microchannel-based　electrical　sensing　technology　with　an　ECL　reporting　system　for　the　first　time.　The　target　regulated　the　effective　radius　and　charge　density　of　a　microchannel　which　in　turn　adjusted　the　ionic　transport　in　it　and　finally　caused　the　change　of　ECL　reporting　of　a　tris(1,10-phenanthroline)ruthenium(ii)/tripropylamine　system.　The　developed　system　has　already　been　applied　to　detect　aflatoxin　B1　for　demonstration.　This　configuration　separated　the　target　sensing　and　reporting　reactions　to　achieve　direct　regulation　of　ECL　reporting　by　ionic　current　and　expanded　the　application　of　the　ECL　detection　technology　to　microanalysis.　©　The　Royal　Society　of　Chemistry　2021.