Wounds　may　remain　open　for　a　few　weeks　in　severe　burns,　which　provide　an　entry　point　for　pathogens　and　microorganisms　invading.　Thus,　wound　dressings　with　long-term　antimicrobial　activity　are　crucial　for　severe　burn　wound　healing.　Here,　a　sandwich　structure　composite　wound　dressing　anchored　with　silver　nanoparticles　(AgNPs)　was　developed　for　severe　burn　wound　healing.　AgNPs　were　in　situ　synthesized　on　the　fibers　of　chitosan　nonwoven　fabric　(CSNWF)　as　the　interlayer　of　wound　dressing　for　sustained　release　of　silver　ion.　The　firmly　anchored　AgNPs　could　prevent　its　entry　into　the　body,　thereby　eliminating　the　toxicity　of　nanomaterials.　The　outer　layer　was　a　polyurethane　membrane,　which　has　a　nanoporous　structure　that　could　maintain　free　transmission　of　water　vapor.　Chitosan/collagen　sponge　was　selected　as　the　inner　layer　because　of　its　excellent　biocompatibility　and　biodegradability.　The　presence　of　AgNPs　in　the　CSNWF　was　fully　characterized,　and　the　high　antibacterial　activity　of　CSNWF/AgNPs　was　confirmed　by　against　Escherichia　coli,　Pseudomonas　aeruginosa　and　Staphylococcus　aureus.　The　superior　wound　healing　effect　on　deep　dermal　burns　of　presented　composite　wound　dressing　was　demonstrated　in　a　porcine　model.　Our　finding　suggested　that　the　prepared　AgNPs　doped　sandwich　structure　composite　wound　dressing　has　great　potential　application　in　severe　wound　care.　©　The　Author(s)　2021.　Published　by　Oxford　University　Press.