Effective　coverage　and　protection　is　a　priority　in　wound　treatment.　Collagen　and　chitosan　have　been　widely　used　for　wound　dressings　due　to　their　excellent　biological　activity　and　biocompatibility.　Silver　nanoparticles　(AgNPs)　have　a　powerful　antibacterial　effect.　In　this　study,　a　macromolecular　and　small-molecular　collagen　mixed　solution,　a　macromolecular　and　small-molecular　chitosan　mixed　solution　were　prepared,　and　a　silver　nanoparticle-loaded　collagen-chitosan　dressing　(AgNP-CCD)　has　been　proposed.　First,　the　effects　of　a　collagen-chitosan　mixed　solution　on　the　proliferation　of　human　umbilical　vein　endothelial　cells　and　the　secretion　of　cytokines　were　evaluated.　Then,　the　characteristics　and　antibacterial　effects　of　the　AgNP-CCD　were　tested,　and　the　effects　on　wound　healing　and　the　influence　of　wound　cytokine　expression　were　investigated　via　a　deep　second-degree　burn　wound　model.　The　results　showed　that　at　the　proper　proportion　and　concentration,　the　collagen-chitosan　mixed　solution　effectively　promoted　cell　proliferation　and　regulated　the　levels　of　growth　factors　(vascular　endothelial　growth　factor　[VEGF],　epidermal　growth　factor　[EGF],　platelet-derived　growth　factor　[PDGF],　transforming　growth　factor　[TGF-b1],　basic　fibroblastic　growth　factor　[bFGF])　and　inflammatory　factors　(TNF-a,　IL-1b,　IL-6,　IL-8).　Moreover,　AgNP　solutions　at　lower　concentrations　exerted　limited　inhibitory　effects　on　cell　proliferation　and　had　no　effect　on　cytokine　secretion.　The　AgNP-CCD　demonstrated　satisfactory　morphological　and　physical　properties　as　well　as　efficient　antibacterial　activities.　An　in　vivo　evaluation　indicated　that　AgNP-CCD　could　accelerate　the　healing　process　of　deep　second-degree　burn　wounds　and　played　an　important　role　in　the　regulation　of　growth　and　inflammatory　factors,　including　VEGF,　EGFL-7,　TGF-b1,　bFGF,　TNF-a　and　IL-1b.　This　AgNP-CCD　exerted　excellent　biological　effects　on　wound　healing　promotion　and　cytokine　expression　regulation.　©　The　Author(s)　2020.