Simple　Summary　Karst　tiankeng　is　a　special　and　magnificent　surface　negative　terrain　with　unique　scientific　value.　The　underground　forests　developed　on　inverted　stone　slopes　in　tiankeng　are　important　areas　for　biodiversity　conservation.　This　research　used　Illumina　high-throughput　sequencing　technology　to　determine　the　soil　microbial　communities　at　four　sites　(at　the　bottom　of　the　slope　(BS),　in　the　middle　of　the　slope　(MS),　in　the　upper　part　of　the　slope　(US),　and　outside　the　tiankeng　(OT))　along　the　inverted　stone　slopes.　The　microbial　communities　at　different　slope　positions　presented　similar　compositions　but　different　abundances.　The　dominant　phyla　in　the　inverted　stone　slope　were　Proteobacteria,　Actinobacteria　and　Acidobacteria.　The　microbial　community　diversity　was　greater　at　the　US　site.　The　microbial　communities　with　more　abundant　functional　genes　involved　in　C/N　cycles　were　located　at　the　BS　site.　The　distribution　of　the　microbial　community　was　highly　correlated　with　the　Total　nitrogen　and　pH.　Understanding　the　soil　microbial　communities　on　inverted　stone　slopes　is　important　for　monitoring　the　ecology　of　tiankeng　and　biodiversity　value　assessments.　The　underground　forests　developed　on　inverted　stone　slopes　in　degraded　karst　tiankengs　are　important　areas　for　biodiversity　conservation,　but　the　microbial　community　profiles　have　not　been　sufficiently　characterized.　Thus,　we　investigated　the　soil　microbial　communities　at　four　sites　(at　the　bottom　of　the　slope　(BS),　in　the　middle　of　the　slope　(MS),　in　the　upper　part　of　the　slope　(US)　and　outside　the　tiankeng　(OT))　in　the　Shenxiantang　tiankeng.　The　dominant　phyla　in　the　inverted　stone　slope　were　Proteobacteria,　Actinobacteria,　and　Acidobacteria,　and　the　relative　abundance　were　different　in　different　slope　positions.　The　Shannon-Wiener　diversity　index　of　the　microbial　community　was　significantly　greater　for　the　US　site　than　for　the　MS　or　BS　sites.　The　metabolism　functional　pathways　(including　C/N　cycle)　were　more　abundant　at　the　BS　site.　Total　nitrogen　and　pH　were　the　dominant　factors　in　determining　the　distribution　of　the　microbial　community　along　an　inverted　stone　slope.　These　results　suggest　that　topographic　heterogeneity　can　influence　the　variations　in　the　soil　microbial　structure,　diversity,　and　function　in　degraded　karst　tiankengs　and　emphasized　the　ecological　value　of　inverted　stone　slopes　within　karst　tiankengs.