Dsk2　is　a　nuclear-enriched　ubiquitin-like　polyubiquitin-binding　protein　that　regulates　protein　degradation　in　yeast　but　has　not　been　explored　yet　in　filamentous　fungi,　such　as　Beauveria　bassiana.　Here,　we　report　Beauveria　bassiana　Dsk2　located　both　in　the　nucleus　and　in　cytoplasm　of　hyphal　cells.　Deletion　of　Dsk2　resulted　in　mild　growth　defect　on　scant　media　with　various　carbon/nitrogen　sources　and　dramatic　attenuation　in　conidiation　capability　at　optimal　condition.　Compared　to　the　wild-type,　Delta　Dsk2　strains　are　much　more　sensitive　to　high　osmotic　and　oxidative　pressure　during　vegetative　growth.　Meanwhile,　the　mutant　strains　showed　an　increased　chemical　tolerance　to　Congo　red　and　calcofluor　white,　two　cell　wall　perturbing　agents.　The　transcriptional　changes　of　genes　involved　in　central　development,　superoxide　dismutase　and　chitin　synthesis　pathway　indicate　that　Dsk2　acts　as　a　multi-functional　regulator　in　adapting　to　environmental　changes.　Importantly,　Dsk2　negatively　regulated　the　ability　of　thermal　resistance　in　B.　bassiana,　which　makes　it　a　potential　target　gene　for　constructing　engineering　anti-thermal　strains　in　the　circumstance　of　global　warming.　Altogether,　our　finding　highlights　novel　roles　of　Dsk2　involved　in　the　asexual　cycle,　multi-stress　tolerance　and　pest　control　potential　of　B.　bassiana.