Home>Results

  • Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

[期刊论文]

Ti4+-immobilized hierarchically porous zirconium-organic frameworks for highly efficient enrichment of phosphopeptides

Share
Edit Delete 报错

author:

He, Yanting (He, Yanting.) [1] | Zheng, Qiong (Zheng, Qiong.) [2] | Lin, Zian (Lin, Zian.) [3] (Scholars:林子俺)

Indexed by:

SCIE

Abstract:

Ti4+-immobilized hierarchically porous zirconium-organic frameworks (denoted as THZr-MOFs) was prepared for phosphopeptide enrichment. The THZr-MOFs showed high specific surface area of 185.28 m(2) g(-1), wide pore-size distribution of 3 similar to 20 nm, good chemical stability and excellent hydrophilicity. Introduction of hierarchical pores in MOFs not only facilitated the accessibility of phosphopeptides to the internal metal affinity sites and reduce their mass transfer resistance, but also increased the exposure sites of metal affinity interaction and binding energies of Zr and Ti elements. Benefited from these advantages, the THZr-MOFs showed high adsorption capacity (79.8 mu g mg(-1)) towards standard phosphopeptide. A low detection limit (0.05 fmol mu L-1) and high enrichment selectivity (beta-casein/BSA with a molar ratio of 1:5000) were also obtained by MALDI-TOF MS. The THZr-MOFs were applied to analyze complex samples including nonfat milk, human serum, and HeLa cell lysate. In total, 1432 phosphopeptides derived from 762 phosphoproteins were identified from human HeLa cell lysate.

Keyword:

HeLa cell Hierarchical pore MALDI-TOF MS Metal-organic frameworks Phosphopeptides Ti4+-immobilization

Community:

  • [ 1 ] [He, Yanting]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Coll Chem, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China
  • [ 2 ] [Zheng, Qiong]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Coll Chem, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China
  • [ 3 ] [Lin, Zian]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Coll Chem, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China

Reprint 's Address:

  • 林子俺

    [Lin, Zian]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Coll Chem, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China

Show more details

Source :

MICROCHIMICA ACTA

ISSN: 0026-3672

Year: 2021

Issue: 5

Volume: 188

6 . 4 0 8

JCR@2021

5 . 4 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:117

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 14

SCOPUS Cited Count: 15

30 Days PV: 0

Online/Total:114/10044125
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1