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author:

林洪宝 (林洪宝.) [1] | 傅蓉 (傅蓉.) [2] | 薛伟明 (薛伟明.) [3] | 郭春腾 (郭春腾.) [4] | 饶平凡 (饶平凡.) [5]

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CQVIP CSCD

Abstract:

使用硫酸铵分级沉淀、 CM-Sephadex C-50弱阳离子、 POROS PE疏水层析和POROS HQ20强阴离子交换分离技术, 从黑曲酶发酵的果胶酶粗品中纯化出一种电泳纯的果胶酶. 经SDS-PAGE电泳测定其相对分子量约41.8kD, 最适pH和最适温度分别为5.0和36℃.

Keyword:

POROS疏水层析 内切聚半乳糖醛酸酶 分离 发酵生产 果胶酶 硫酸铵分级沉淀 纯化 黑曲霉

Community:

  • [ 1 ] 福州大学生物工程研究所,福建,福州,350002

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Source :

福州大学学报:自然科学版

ISSN: 1000-2243

CN: 35-1337/N

Year: 2002

Issue: 5

Volume: 30

Page: 632-635

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 0

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