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author:

谢秀气 (谢秀气.) [1] | 王中来 (王中来.) [2] | (等.) [3]

Indexed by:

CQVIP CSCD

Abstract:

采用沉降脱脂分离技术,从鸡超免疫卵黄中提取传染性腔上囊病卵黄抗体免疫球蛋白(IGY)。通过正交试验,确定乙酸-乙酸钠缓冲液为最佳沉降脱脂缓冲液。用PH值为5.2,浓度为0.12mol/L的乙酸-乙酸钠缓冲液9倍稀释卵黄液,在约300r/min下搅拌10min后,置于4℃下沉降3-4h,沉降上清液中IgY含量占卵黄中蛋白总量的25%左右,脱酯率>98%,活性损失可忽略不计。

Keyword:

传染性腔上囊病 制备工艺 卵黄抗体 沉降脱脂条件

Community:

  • [ 1 ] 福州大学生物工程研究所,福建福州250002

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Source :

中国兽医科技

ISSN: 1000-6419

Year: 2002

Issue: 2

Volume: 32

Page: 25-26

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 5

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