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Abstract:
采用沉降脱脂分离技术,从鸡超免疫卵黄中提取传染性腔上囊病卵黄抗体免疫球蛋白(IGY)。通过正交试验,确定乙酸-乙酸钠缓冲液为最佳沉降脱脂缓冲液。用PH值为5.2,浓度为0.12mol/L的乙酸-乙酸钠缓冲液9倍稀释卵黄液,在约300r/min下搅拌10min后,置于4℃下沉降3-4h,沉降上清液中IgY含量占卵黄中蛋白总量的25%左右,脱酯率>98%,活性损失可忽略不计。
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中国兽医科技
ISSN: 1000-6419
Year: 2002
Issue: 2
Volume: 32
Page: 25-26
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count: -1
30 Days PV: 5
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