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author:

朱碧银 (朱碧银.) [1] | 洪文荣 (洪文荣.) [2] | 李辉 (李辉.) [3]

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CQVIP PKU CSCD

Abstract:

以研究黑暗链霉菌tt-49中生物合成基因aprf-g生理功能为目标,利用接合转移法,将同源重组质粒pfd10导入黑暗链霉菌tt-49,最终获得同源双交换茵株tt-49g53,并对其生理特性进行考察.结果显示:其生长速度明显比亲株慢,产抗水平比亲株低100倍,仅为13 u/ml.采用薄层层析法对其代谢产物进行分析,未检测到安普霉素.

Keyword:

分子遗传工程 基因重组 黑暗链霉菌

Community:

  • [ 1 ] 福州大学生物科学与工程学院,福建福州,350108

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Source :

食品与生物技术学报

ISSN: 1673-1689

Year: 2012

Issue: 4

Page: 391-395

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 0

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