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author:

朱碧银 (朱碧银.) [1] | 洪文荣 (洪文荣.) [2] | 李辉 (李辉.) [3]

Indexed by:

PKU CSCD

Abstract:

以研究黑暗链霉菌Tt-49中生物合成基因aprF-G生理功能为目标,利用接合转移法,将同源重组质粒pFD10导入黑暗链霉菌Tt-49,最终获得同源双交换菌株Tt-49G53,并对其生理特性进行考察。结果显示:其生长速度明显比亲株慢,产抗水平比亲株低100倍,仅为13U/mL。采用薄层层析法对其代谢产物进行分析,未检测到安普霉素。

Keyword:

分子遗传工程 基因重组 黑暗链霉菌

Community:

  • [ 1 ] 福州大学生物科学与工程学院

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Source :

食品与生物技术学报

Year: 2012

Issue: 04

Volume: 31

Page: 391-395

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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