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目的:从西索霉素产生菌中克隆出表达西索米星的糖基转移酶基因sisZ.方法:采用PCR法从伊纽小单孢菌(Micromonospora inyoensis)的染色体扩增参与西索霉素生物合成的糖基转移酶基因sisZ,然后先后装载到克隆载体pUC18和表达载体pET-30a上.结果和结论:成功从伊纽小单孢菌扩增出参与西索霉素生物合成的糖基转移酶基因sisZ.其开放阅读框长1176bp,编码含391个氨基酸(41690 D)的多肽链,并在大肠杆菌E. coli BL21 (DE3)中实现表达.基因sisZ的碱基序列与gntZ (M. echinospora)的碱基序列的同源性高达94%.预测的SisZ蛋白序列与GntZ的同源性为98%.
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中国药科大学学报
ISSN: 1000-5048
CN: 32-1157/R
Year: 2008
Issue: 3
Volume: 39
Page: 271-273
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count: -1
Chinese Cited Count:
30 Days PV: 6
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