Translated Title
Optimization of the production of (r)-α-hydroxybenzeneacetic acid by Bacillus sp.HB20 in ionic liquid containing systems
Translated Abstract
(R)-α-Hydroxyphenylacetic acid ((R)-HPA) was an important drug intermediates. In the current study, Bacillus sp.HB20 was used to catalyze the reduction of phenylglyoxylic acid (PGA) to (R)-HPA in the system containing ionic liquid ([bmim]Cl) and response surface methodology was successfully used to optimize the production of (R)-HPA. A significant influence of ionic liquid (IL) content, reaction temperature, and PGA concentration on (R)-HPA production was noted with Plackett-Burman design and the steepest ascent was applied to approach the optimal response area. Then, a three-level Box-Behnken design was employed to optimize the culture conditions for the production of (R)-HPA. The optimal conditions to achieve the optimal (R)-HPA production were determined: IL content, 34.3 g/L; reaction temperature, 33 ℃;PGA concentration, 15.3 mmol/L. The maximum production of (R)-HPA was 82.39%, which was about 21.66%increase compared with that obtained from the original culture conditions. It can be concluded that using response surface methodology to improve the (R)-HPA yield is available. It provides a new green method for the industrial production of chiral drugs.
Translated Keyword
biotransformation
ionic liquid
Plackett-Burman
response surface methodology
(R)-α-Hydroxyphenylacetic acid
Access Number
WF:perioarticaljsjyyyhx201306006
