• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

程欲 (程欲.) [1] | 缪少锋 (缪少锋.) [2] | 李仁宽 (李仁宽.) [3] | 陈菁 (陈菁.) [4] | 刘树滔 (刘树滔.) [5] (Scholars:刘树滔) | 饶平凡 (饶平凡.) [6]

Indexed by:

CQVIP PKU CSCD

Abstract:

将尖吻蝮蛇类凝血酶基因克隆到载体pET32a(+)上,在大肠杆菌Rosetta2(DE3)中表达N端融合了硫氧还原蛋白的类凝血酶.通过SDS-PAGE检测融合蛋白条带,纤维蛋白原凝结实验检测酶学活性.确定表达菌株后,对其诱导条件进行优化,在30℃诱导3h,诱导剂IPTG浓度为0.5 mmol·L-1条件下类凝血酶表达水平最高.

Keyword:

acutobin 原核表达 尖吻蝮蛇 类凝血酶

Community:

  • [ 1 ] [程欲]福州大学
  • [ 2 ] [缪少锋]福州大学
  • [ 3 ] [李仁宽]福州大学
  • [ 4 ] [陈菁]福州大学
  • [ 5 ] [刘树滔]福州大学
  • [ 6 ] [饶平凡]福州大学

Reprint 's Address:

Email:

Show more details

Version:

Related Keywords:

Source :

福州大学学报(自然科学版)

ISSN: 1000-2243

CN: 35-1337/N

Year: 2013

Issue: 2

Volume: 41

Page: 252-256

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count: -1

Chinese Cited Count:

30 Days PV: 1

Online/Total:81/10016736
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1