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Abstract:
将尖吻蝮蛇类凝血酶基因克隆到载体pET32a(+)上,在大肠杆菌Rosetta2(DE3)中表达N端融合了硫氧还原蛋白的类凝血酶.通过SDS-PAGE检测融合蛋白条带,纤维蛋白原凝结实验检测酶学活性.确定表达菌株后,对其诱导条件进行优化,在30℃诱导3h,诱导剂IPTG浓度为0.5 mmol·L-1条件下类凝血酶表达水平最高.
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福州大学学报(自然科学版)
ISSN: 1000-2243
CN: 35-1337/N
Year: 2013
Issue: 2
Volume: 41
Page: 252-256
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count: -1
Chinese Cited Count:
30 Days PV: 1
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