Translated Title
Screening and breeding by induced mutation of xylanase-producing marine microorganism
Translated Abstract
With self-made xylan as the only carbon source of primary screening medium , totally 60 strains which showed transparent zones and different morphology were screened from different marine samples .The shake flask fermentation results showed that 38 strains had xylanase-producing capabil-ities and strain B659 had the highest xylanase activity of 525.3 U· mL-1 .Via morphological charac-teristics and 16S rDNA sequence analysis , strain B659 was identified as Bacillus.The stable mutant strain G3-17 with 13.9%enhancement of xylanase activity was screened from the UV mutagenesis of strain B659.A stable mutant strain W1-40 with 11.6% xylanase activity increase was obtained by microwave mutation and screening from strain G 3-17.In 72 h fermentation of strain B659 and strain W1-40, the xylanase activity of latter (645.2 U· mL-1 ) achieved 24.6%elevation than the former (517.9 U· mL-1).
Translated Keyword
marine microorganism
mutagenesis
screening
xylanase
Access Number
WF:perioarticalfzdxxb201505025
