Inroduction:Antifungal　proteins　play　an　important　role　in　the　defense　of　crops　against　fungal　infection.Identification　of　those　antifungal　proteins　is　vital　to　the　understanding　of　the　natural　defense　mechanism　of　plants　and　crops.It　can　also　provide　useful　clues　in　the　development　of　biologically　effective　and　environment-friendly　pest　management　agents　to　replace　traditional　chemical　pesticides.Our　objective　in　this　study　was　to　isolate　novel　proteins　with　antifungal　activity　from　black　soybean.