To　prepare　and　characterize　the　mouse　monoclonal　antibodies　against　Vibrio　parahaemolyticus　OmpW.　The　OmpW　amino　acid　sequence　from　three　diseased　Vibrio　was　analyzed　by　Bioinformatics.　Mice　were　immunized　with　r-OmpW　which　was　highly　expressed　and　purified　in　E.coli.　Five　Vibrio(Va,　Vp,　Vh,　Vv,　Van)　were　chosen　as　antigen　for　mAb　selection.The　characters　of　the　anti-OmpW　monoclonal　antibodies　were　studied　by　Western　blot,　Flow　Cytometry,　indirect　immunofluorescence.　OmpW　was　testified　a　highly　conservative　membrane　protein.Three　clones　of　anti-OmpW　mAb　was　obtained.　The　Ig　subclass　of　the　mAb　secreted　from　fused　cell　S5C10　was　IgG3,　which　of　the　titer　was　4.6×10(4);.　The　mAb　could　specifically　recognize　Vibrio　parahaemolyticus,　Vibrio　alginolyticus,　Vibrio　harveyi,　Vibrio　anguillarum,　Vibrio　vulnificus,　which　could　not　react　with　Pseudomonas　flurosecens,　Aeromonas　hydrophila,　Aeromonas　sobria,　Aeromonas　hydrophila,　Escherichia　coli.　The　mAb　could　specially　recognize　five　diseased　Vibrio,　which　is　a　useful　tool　for　the　further　study　of　the　diagnosis　of　Vibrio.