The　simulation　of　the　light-guide　structure　of　the　edge-lit　LED　backlight　source　and　the　influence　of　the　practical　factors　on　the　light　source　are　studied.　Firstly,　the　main　functions　and　characteristics　of　the　edge-lit　backlight　light　structure　are　briefly　analyzed,　and　the　corresponding　theoretical　basis　is　provided.　Then,　a　simplified　and　accurate　light　guide　structure　simulation　model　is　proposed　based　on　the　optical　characteristics　of　light-guide　structure,　and　the　model　is　simulated　with　the　optical　software　of　TracePro.　Finally,　an　in-depth　discussion　and　analysis　on　the　characteristics　and　influences　of　light-guide　structure　of　the　LED　backlight　source　is　presented　with　the　actual　processing　practice.　The　simulation　results　are　verified　by　corresponding　experiments.　The　influence　of　the　essential　factors　on　the　effect　of　the　backlight　source,　including　the　matching　between　the　light　guide　structure　and　the　LED　light　source,　making　the　light　guide　plate　thinner　and　designing　dot　scheme　for　high　efficiency　and　good　uniformity,　is　emphatically　analyzed　for　LED　backlight　system.　This　research　may　offer　some　practical　significance,　for　the　reason　that　it　proposes　a　reasonable　and　optimized　design　for　light　guide　parameters　and　alleviates　potential　mistakes　in　the　process　of　actual　production.　©　2016,　Editorial　Office　of　Opto-Electronic　Engineering.　All　right　reserved.