The　effects　of　temperature,　local　tap-water　and　culture　medium　without　sterilization　on　cell　growth　and　lutein　production　of　Desmodesmus　sp.　F51　were　investigated　under　indoor　conditions.　Outdoor　batch　cultivation　of　Desmodesmus　sp.　F51　was　also　investigated　in　50　L　cylindrical　photobioreactor.　The　results　showed　that　the　best　biomass　productivity　[767.5　mg/(L·d)]　and　lutein　productivity　[3.05　mg/(L·d)]　occurred　at　a　temperature　of　35℃.　A　low　temperature　enhanced　the　lutein　content　but　reduced　cell　growth　rate.　There　is　no　significant　growth　inhibition　and　microbiological　contamination　were　observed　for　Desmodesmus　sp.　F51　using　local　tap-water　and　culture　medium　without　sterilization.　Under　the　outdoor　temperature　of　13.0~27.6℃,　the　highest　biomass　productivity,　CO2　fixation　rate　and　lutein　productivity　of　236.3,　444.2　and　1.17　mg/(L·d)　were　obtained,　respectively,.　©　2016,　Science　Press.　All　right　reserved.