The　exchange　capacity　and　desorption　profiles　of　sisomicin　in　some　macroporous　carboxylic　acid　type　ion　exchange　resins　and　the　conventional　sulfonic　acid　type　ion　exchange　resin　were　compared.　Primary　experiments　were　conducted　in　three　kinds　of　carboxylic　acid　type　ion　exchange　resins　(Resin　I,　II,　III),　and　Resin　I　was　selected　for　further　study,　and　compared　the　specific　characteristics　with　the　sulfonic　acid　type　resin　732.　Results　showed　that　the　static　exchange　capacity　of　Resin-　I　to　sisomicin　was　18.　1×107　μg/ml,　which　was　80%　higher　than　that　of　sulfonic　acid　type　resin　732.　The　optimum　pH　for　adsorption　of　sisomicin　was　around　7.5;　Dynamic　exchange　capacity　of　Resin-　I　was　further　increased　by　13.8%　than　static　exchange　capacity.　In　dynamic　exchange　of　extraction　and　desorption　of　sisomicin,　the　total　recovery　rate　was　82.6%,　and　it　was　8%　higher　than　using　the　sulfonic　acid　type　resin　732.　The　elution　of　sisomicin　was　performed　by　use　of　5%　aqueous　ammonia.　The　elution　profile　showed　that　the　curve　peak　of　Resin　I　was　more　concentrate　and　less　tailing　than　the　sulfonic　acid　type　resin　732.