Non-Hodgkin＇s　lymphoma　(NHL)　is　the　most　common　adult　hematological　cancer.　With　the　advent　of　combination　therapy　of　chemotherapy　and　the　monoclonal　anti-CD20　antibody　Rituximab,　the　substantial　advancement　in　the　treatment　of　B-cell　malignancies　has　been　achieved.　In　the　clinical　treatment　of　NHL,　however,　there　are　still　many　patients　who　are　not　sensitive　to　the　therapy　of　rituximab.　Hence　investigating　the　interactions　between　rituximab　and　lymphoma　cells　is　crucial　for　us　to　understand　the　actions　of　rituximab　and　design　drugs　with　better　efficacies.　Traditional　biochemical　methods　for　cell　detection　require　the　various　pretreatments　of　the　cell,　destroying　the　structures　of　cells.　This　paper　uses　atomic　force　microscopy　(AFM)　to　label-free　characterize　the　micro/nano　physical　properties　of　single　lymphoma　cells,　including　cell　morphology,　cell　elasticity,　and　molecular　interactions　on　the　cell　surface.　The　study　improved　our　understanding　of　the　rituximab　actions.　©　2017　IEEE.