This　paper　describes　the　oxidative　dissolution　kinetics　of　sulfides　with　gold　occlusion　within　pyrite　and　arsenopyrite.　Shake　flasks　tests　and　column　leaching　of　a　low　grade　gold　ore　from　China　were　carried　out　with　domesticated　mixed　acidophiles　isolated　from　acid　mine　drainage.　Both　test　show　that　the　main　factors　accelerating　sulfide　oxidation　was　mainly　temperature　and　redox　potential.　Column　bio-oxidation　of　mineral　with　a　particle　size　less　than　10　mm　at　60°C　resulted　in　higher　mineral　decomposition,　finer　fractions　and　eventually　higher　sulfide　oxidation　than　that　at　30°C.　Sulfide-S　dissolution　increased　from　58%　to　77%　from　30°C　to　60°C　after　247　ds　of　biooxidation.　Further　investigation　into　microbial　community　attached　to　the　ore　surface　and　in　the　leachate　during　the　bio-oxidation　was　done　by　Real-time　PCR　assays.　Organism　of　genera　Acidithiobacillus　was　the　most　dominant　species　in　both　leachate　and　ore　surface　at　lower　temperature.　For　the　Archaea,　the　iron　oxidizing　microbial　Ferroplasma　showed　its　predominance　of　60°C.　Mineral　dissolution　kinetics　and　microbial　community　in　bio-oxidation　was　lucubrated　in　this　work　and　suggestions　were　provided　for　pre-treatment　of　refractory　gold　ore.　©　2018　Trans　Tech　Publications,　Switzerland.