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Abstract:
The kinetics of asymmetric production of R-(-)-mandelic acid (R-MA) from phenylglyoxylic acid (PGA) catalyzed by Saccharomyces cerevisiae sp. strain FD11b was studied by fed-batch cultures. The concentrations of glucose and PGA were controlled respectively with a dual feeding system. When the electron donor glucose was supplied at the rate of 0.0833 mmol·gdw-1·h-1, the specific production rate (qp) and the enantiomeric excess of R-MA reached the maximum 0.353 mmol·gdw-1·-1 and 97.1%, respectively. The apparent reduction activity of yeast FD11b was obviously affected by both substrate PGA and product MA. The qp value reached the maximum 0.36-0.38 mmol·gdw-1·h-1 when the PGA concentration was controlled between 25 and 35 mmol·L-1. The obvious substrate inhibition of bioconversion was observed at the PGA concentrations higher than 40 mmol·L-1. The accumulation of product MA also caused a severe feed-back inhibition for its production when the product concentration was above 60 mmol·L-1. The kinetic model with the inhibition effect of both substrate and product was simulated by a computer-based least-square arithmetic. The established kinetic model was in good agreement with the experimental data.
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Chinese Journal of Chemical Engineering
ISSN: 1004-9541
Year: 2006
Issue: 1
Volume: 14
Page: 73-80
0 . 3 9 3
JCR@2006
3 . 7 0 0
JCR@2023
JCR Journal Grade:3
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ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
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30 Days PV: 1
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