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author:

Xiao, Meitian (Xiao, Meitian.) [1] | Huang, Yayan (Huang, Yayan.) [2] | Meng, Chun (Meng, Chun.) [3] | Guo, Yanghao (Guo, Yanghao.) [4]

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Abstract:

The kinetics of asymmetric production of R-(-)-mandelic acid (R-MA) from phenylglyoxylic acid (PGA) catalyzed by Saccharomyces cerevisiae sp. strain FD11b was studied by fed-batch cultures. The concentrations of glucose and PGA were controlled respectively with a dual feeding system. When the electron donor glucose was supplied at the rate of 0.0833 mmol·gdw-1·h-1, the specific production rate (qp) and the enantiomeric excess of R-MA reached the maximum 0.353 mmol·gdw-1·-1 and 97.1%, respectively. The apparent reduction activity of yeast FD11b was obviously affected by both substrate PGA and product MA. The qp value reached the maximum 0.36-0.38 mmol·gdw-1·h-1 when the PGA concentration was controlled between 25 and 35 mmol·L-1. The obvious substrate inhibition of bioconversion was observed at the PGA concentrations higher than 40 mmol·L-1. The accumulation of product MA also caused a severe feed-back inhibition for its production when the product concentration was above 60 mmol·L-1. The kinetic model with the inhibition effect of both substrate and product was simulated by a computer-based least-square arithmetic. The established kinetic model was in good agreement with the experimental data.

Keyword:

Bioconversion Biomedical engineering Catalyst activity Computer simulation Glucose Mathematical models Organic acids Reaction kinetics Reduction Substrates

Community:

  • [ 1 ] [Xiao, Meitian]Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350001, China
  • [ 2 ] [Xiao, Meitian]Institute of Pharmaceutical Engineering, Huaqiao University, Quanzhou 362021, China
  • [ 3 ] [Huang, Yayan]Institute of Pharmaceutical Engineering, Huaqiao University, Quanzhou 362021, China
  • [ 4 ] [Meng, Chun]Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350001, China
  • [ 5 ] [Guo, Yanghao]Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350001, China

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Source :

Chinese Journal of Chemical Engineering

ISSN: 1004-9541

Year: 2006

Issue: 1

Volume: 14

Page: 73-80

0 . 3 9 3

JCR@2006

3 . 7 0 0

JCR@2023

JCR Journal Grade:3

Cited Count:

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ESI Highly Cited Papers on the List: 0 Unfold All

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Chinese Cited Count:

30 Days PV: 1

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