A　porous　polymethacrylate　ester-based　monolithic　column　for　capillary　electrochromatography　(CEC)　was　designed　by　mean　of　in　situ　co-polymerizing　lauryl　methacrylate　(LMA),　ethylene　dimethacrylate　(EDMA)　and　2-acrylamido-2-methyl-1-propanesulfonic　acid　(AMPS)　in　a　ternary　porogenic　solvent　including　cyclohexanol,　1,4-butanediol　and　water.　After　investigating　the　influence　factors　of　the　CEC　monolithic　columns,　four　flavonoids　(i.e.,　Rutin,　Quercetin,　Kaempferol,　and　Quercitrin)　were　separated　and　assayed　to　evaluate　this　monolithic　column　with　CEC　method.　Under　optimum　conditions,　the　CEC　method　exhibited　high　separation　efficiency,　with　rapid　separation　time　of　3-4.　min,　for　the　four　flavonoid　samples　using　10.　mM　phosphate　buffer　containing　70%　acetonitrile　(pH　9.0).　Importantly,　the　proposed　method　could　provide　a　promising　approach　for　rapid　separation　and　detection　in　biomedicine.　©　2010.