A　trypsin-chymotrypsin　inhibitor,　designated　Limenin,　with　both　antifungal　and　antibacterial　activity,　and　exhibiting　a　molecular　mass　of　18.0　kDa　in　SDS-polyacrylamide　gel　electrophoresis,　was　isolated　from　the　large　lima　bean　(Phaseolus　limensis)　legumes　by　a　combination　of　extraction,　ammonium　sulfate　precipitation,　ion　exchange　chromatography　on　SP-Toyopearl　and　high　performance　liquid　chromatography　(HPLC)　on　Mono　S.　The　isoelectric　point　was　estimated　to　be　7.6　by　isoelectric　focusing.　The　15　N-terminal　amino　acid　sequences　were　determined　to　be　DFVIDNEGNPLENGG,　demonstrating　some　resemblance　to　those　other　protease　inhibitors　and　inhibitor　precursors　from　leguminous　plants.　It　exerted　potent　antifungal　action　toward　Botrytis　cinerea,　Alternaria　alternata(Fr.)　Keissl,　and　Pythium　aphanidermatum.　It　showed　antiproliferative　activity　toward　tumor　cells　including　human　liver　hepatoma　cells　Bel-7402　and　neuroblastoma　cells　SHSY5Y.　However,　it　had　no　effect　on　bacteria　Staphylococcus　aureus　and　Salmonella.　©　Springer-Verlag　2010.