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A highly sensitive and selective electrochemiluminescent (ECL) biosensor for the determination of adenosine was developed. Single DNA (capture DNA) was immobilized on the gold electrode through Au-thiol interaction at first. Another DNA modified with tris(2,2'-bipyridyl) ruthenium(II)-doped silica nanoparticles (Ru-SNPs) that contained adenosine aptamer was then modified on the electrode surface through hybridizing with the capture DNA. In the presence of adenosine, adenosine-aptamer complex is produced rather than aptamer-DNA duplex, resulting with the dissociation of Ru-SNPs-labeled aptamer from the electrode surface and the decrease in the ECL intensity. The decrease of ECL intensity has a direct relationship with the logarithm of adenosine concentration in the range of 1.0×10-10 to 5.0×10-6molL-1. The detection limit of the proposed method is 3.0×10-11molL-1. The existence of guanosine, cytidine and uridine has little interference with adenosine detection, demonstrating that the developed biosensor owns a high selectivity to adenosine. In addition, the developed biosensor also demonstrates very good reusability, as after being reused for 30 times, its ECL signal still keeps 91% of its original state. © 2010 Elsevier B.V.
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Analytica Chimica Acta
ISSN: 0003-2670
Year: 2011
Issue: 1-2
Volume: 684
Page: 121-125
4 . 5 5 5
JCR@2011
5 . 7 0 0
JCR@2023
JCR Journal Grade:1
CAS Journal Grade:2
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